By means of molecular imprinting of a conducting polymer, molecular cavities selective for oxytocin nonapeptide, an autism biomarker, were designed. Embedding of the oxytocin template, and then its extracting from the molecularly imprinted polymer (MIP) was confirmed by the XPS analysis. AFM imaging of the MIP film surface indicated changes in mechanical properties of the film after template extraction. The MIP synthetic receptor was deposited by potentiodynamic electropolymerization as a thin film on an Au film electrode in an electrochemical miniaturized microfluidic cell. The use of this cell allowed to shorten analysis time and to decrease the sample volume. The linear dynamic concentration range extended from 0.06 to 1 mM with the limit of detection of 60 µM (S/N = 3). Advantageously, sensitivity of the diagnostic microfluidic platform devised for oxytocin determination in both synthetic serum samples and in aqueous solutions was similar and, moreover, it was selective to common interferences, such as oxytocin analogs and potential metabolites.

通过导电聚合物的分子印迹，设计了对催产素九肽具有选择性的分子腔，这是一种自闭症生物标志物。嵌入催产素模板，然后通过XPS分析确认其是从分子印迹聚合物（MIP）中提取的。MIP薄膜表面的AFM成像表明模板提取后薄膜的机械性能发生了变化。通过电势电聚合将MIP合成受体以薄膜形式沉积在电化学微型化微流电池中的Au膜电极上。使用该样品池可以缩短分析时间并减少样品量。线性动态浓度范围从0.06扩展到1 mM，检出限为60 µM（S / N = 3）。有利地，