In this article, blue-emitting carbon dots (CDs) were prepared via hydrothermal treatment of sodium citrate and NH₄HCO₃, and then combined with 3-aminophenylboronic acid (APBA) to prepare APBA modified-CDs. APBA acted as the receptor of dopamine (DA). Using bovine serum albumin (BSA) as a stabilizer and N₂H₄·H₂O as a reducing reagent, BSA-stabilized and red-emitting copper nanoclusters (CuNCs) were prepared. By carbodiimide-activated coupling, novel nanohybrids consisting of CDs and CuNCs were constructed and exhibited dual-emitting fluorescence (FL). In the presence of DA, marked FL (at 440 nm) quenching of nanohybrids was detected. Specific coupling interactions between boric acid of APBA and cis-glycol of DA induced the combination of DA and APBA on the surface of CDs. As a superior electron receptor, DA triggered the electron transfer from CDs to DA, resulting in the FL quenching of CDs in nanohybrids. The FL (at 640 nm) of CuNCs in nanohybrids was almost unchanged after the addition of DA, and so further used for a reference FL to develop a novel ratiometric FL probe for DA detection. In addition to high sensitivity and selectivity, superior analytical performances of this probe were confirmed in applications, including dual-signal FL sensing of DA and naked-eye visual FL imaging of DA in aqueous solution and on filter paper.

本文通过柠檬酸钠和NH ₄ HCO _3的水热处理制备了发蓝光的碳点（CD），然后与3-氨基苯基硼酸（APBA）结合制备APBA修饰的CD。APBA充当多巴胺（DA）的受体。使用牛血清白蛋白（BSA）作为稳定剂和N ₂ H ₄ ·H ₂O作为还原剂，制备了BSA稳定的和发红光的铜纳米团簇（CuNC）。通过碳二亚胺激活的偶联，构建了由CD和CuNC组成的新型纳米杂交体，并显示出双发射荧光（FL）。在DA的存在下，检测到纳米杂化物的明显FL（在440 nm）淬灭。APBA的硼酸与DA的顺式乙二醇之间的特异性偶联相互作用诱导了CD表面上DA和APBA的结合。作为优良的电子受体，DA触发了电子从CD转移到DA，从而导致纳米杂化物中CD的FL猝灭。加入DA后，纳米杂化物中CuNCs的FL（在640 nm）几乎没有变化，因此进一步用作参考FL，以开发用于DA检测的新型比率式FL探针。除了高灵敏度和选择性之外，