In this study, a rapid method for simultaneous detection of ethyl carbamate (EC) and urea in Korean rice wine was developed. To achieve quantitative analysis of EC and urea, the conditions for Ultra-performance liquid chromatography (UPLC) separation and atmospheric-pressure chemical ionization tandem mass spectrometry (APCI-MS/MS) detection were first optimized. Under the established conditions, the detection limit, relative standard deviation and linear range were 2.83 μg/L, 3.75–5.96%, and 0.01–10.0 mg/L, respectively, for urea; the corresponding values were 0.17 μg/L, 1.06–4.01%, and 1.0–50.0 μg/L, respectively, for EC. The correlation between the contents of EC and its precursor urea was determined under specific pH (3.5 and 4.5) and temperature (4, 25, and 50 °C) conditions using the developed method. As a result, EC content was increased with greater temperature and lower pH. In Korean rice wine, urea was detected 0.19–1.37 mg/L and EC was detected 2.0–7.7 μg/L. The method developed in this study, which has the advantages of simplified sample preparation, low detection limits, and good selectivity, was successfully applied for the rapid analysis of EC and urea.

在这项研究中，开发了一种快速检测韩国米酒中氨基甲酸乙酯（EC）和尿素的快速方法。为了实现对EC和尿素的定量分析，首先优化了超高效液相色谱（UPLC）分离和大气压化学电离串联质谱（APCI-MS / MS）检测的条件。在确定的条件下，尿素的检出限，相对标准偏差和线性范围分别为2.83μg/ L，3.75-5.96％和0.01-10.0 mg / L。EC的相应值分别为0.17μg/ L，1.06-4.01％和1.0-50.0μg/ L。使用开发的方法，在特定的pH（3.5和4.5）和温度（4、25和50°C）条件下确定EC及其前体尿素含量之间的相关性。因此，EC含量随温度升高和pH值降低而增加。在韩国米酒中，尿素的含量为0.19–1.37 mg / L，EC含量为2.0–7.7μg/ L。本研究开发的方法具有样品制备简单，检测限低，选择性好等优点，已成功地用于EC和尿素的快速分析。