A new ratiometric fluorescent probe (RPM) was developed based on through-bond energy transfer (TBET) mechanism for detecting hypochlorous acid. The probe was constructed from imidazo[1,5-a]pyridine as the energy donor and the rhodamine as the energy acceptor. The probe could be used to determine the concentration of HOCl with high selectivity and sensitivity (the detection limit = 2.08 × 10⁻⁶ M). In addition, RPM had a good time resolution for measuring HOCl with the response time of less than 30 s. Upon addition of HOCl, the fluorescence colors of probe RPM solution changed from green to red. Moreover, the probe has been successfully utilized for sensing HOCl in living cells.

基于穿透键能量转移（TBET）机理，开发了一种新的比例荧光探针（RPM），用于检测次氯酸。该探针是由咪唑并[1，5- a ]吡啶作为能量供体而若丹明作为能量受体而构建的。该探针可用于高选择性和高灵敏度（检测极限= 2.08×10 ^-6  M），用于确定HOCl的浓度。此外，RPM具有良好的时间分辨率，可用于测量HOCl，响应时间小于30 s。加入HOCl后，探针RPM溶液的荧光颜色从绿色变为红色。而且，该探针已经成功地用于感测活细胞中的HOC1。