Delivery of large and functionally active biomolecules across cell membranes presents a challenge in cell biological experimentation. For this purpose, we developed a novel solid-phase reverse transfection method that is suitable for the intracellular delivery of proteins into mammalian cells with preservation of their function. We show results for diverse application areas of the method, ranging from antibody-mediated inhibition of protein function to CRISPR/Cas9-based gene editing in living cells. Our method enables prefabrication of “ready to transfect” substrates carrying diverse proteins. This allows their easy distribution and standardization of biological assays across different laboratories.

跨细胞膜递送具有功能活性的大生物分子对细胞生物学实验提出了挑战。为此，我们开发了一种新型固相反转染方法，适用于将蛋白质细胞内递送到哺乳动物细胞中并保留其功能。我们展示了该方法不同应用领域的结果，从抗体介导的蛋白质功能抑制到活细胞中基于 CRISPR/Cas9 的基因编辑。我们的方法能够预制携带不同蛋白质的“准备转染”底物。这使得他们可以在不同的实验室轻松分配和标准化生物测定。