The main limitations of lipidomics analysis are the chemical complexity of the lipids, the range of concentrations at which they exist, and the variety of samples usually analyzed. These limitations particularly affect the characterization of polar lipids owing to the interference of neutral lipids, essentially acylglycerides, which are at high concentration and suppress ionization of low concentrated lipids in mass spectrometry detection. The influence of sample preparation on lipidomics analysis of polar lipids in adipose tissue by LC–MS/MS was the aim of this research. Two common extractants used for lipids isolation, methanol:chloroform (MeOH:CHCl₃) and methyl tert-butyl ether (MTBE), were qualitatively and quantitatively compared for the extraction of the main families of lipids. The obtained results showed that each family of lipids is influenced differently by the extractant used. However, as a general trend, the use of MTBE as extractant led to higher extraction efficiency for unsaturated fatty acids, glycerophospholipids and ceramides, while MeOH:CHCl₃ favored the isolation of saturated fatty acids and plasmalogens. The implementation of a solid-phase extraction (SPE) step for selective isolation of glycerophospholipids prior to LC–MS/MS analysis was assayed to evaluate its influence on lipids detection coverage as compared to direct analysis. This step was critical to enhance the detection coverage of glycerophospholipids by removal of ionization suppression effects caused by acylglycerides.

脂质组学分析的主要局限性是脂质的化学复杂性，它们存在的浓度范围以及通常分析的样品种类。由于中性脂质（基本上是酰基甘油酯）的干扰，这些限制特别影响极性脂质的表征，中性脂质处于高浓度并抑制了质谱检测中低浓度脂质的电离。本研究的目的是通过LC-MS / MS对样品制备对脂肪组织中极性脂质的脂质组学分析的影响。两种用于脂质分离的常用萃取剂：甲醇：氯仿（MeOH：CHCl ₃）和甲基叔丁基定性和定量比较了丁基醚（MTBE）提取主要脂质家族。获得的结果表明，所使用的萃取剂对每个脂质家族的影响都不同。然而，总的趋势是，使用MTBE作为萃取剂可提高对不饱和脂肪酸，甘油磷脂和神经酰胺的萃取效率，而MeOH：CHCl ₃赞成分离饱和脂肪酸和缩醛磷脂。在进行LC-MS / MS分析之前，采用固相萃取（SPE）步骤选择性分离甘油磷脂的方法，以评估其与直接分析相比对脂质检测覆盖率的影响。此步骤对于通过消除由酰基甘油酯引起的电离抑制作用来增强甘油磷脂的检测覆盖率至关重要。