A novel and facile ratiometric fluorescence method for evaluating Cu²⁺ has been developed based on coumarin-labeled single-stranded DNA (C-ssDNA) coupled with the Cu²⁺-induced oxidation of o-phenylenediamine (OPD). By combining the microdialysis technique, the ratiometric fluorescence method has also been successfully exploited to monitor the cerebral Cu²⁺ in the rat brain, promising new opportunities for studying the cerebral Cu²⁺-associated physiological and pathological events.

中文翻译：

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比率荧光监测脑的Cu ²⁺基于加上铜香豆素标记的DNA ²⁺的诱导氧化ö苯二胺

基于香豆素标记的单链DNA（C-ssDNA）结合Cu ²⁺诱导的邻苯二胺（OPD）的氧化，开发了一种新颖且简便的比率荧光分析法来评估Cu ²⁺。通过结合微透析技术，比率荧光法已经成功地用于监测大鼠脑中的脑Cu ²⁺，为研究脑Cu ²⁺相关的生理和病理事件提供了新的机会。