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Ratiometric fluorescence monitoring of cerebral Cu2+ based on coumarin-labeled DNA coupled with the Cu2+-induced oxidation of o-phenylenediamine
Analyst ( IF 3.6 ) Pub Date : 2017-08-04 00:00:00 , DOI: 10.1039/c7an01099k Shishi Ma 1, 2, 3, 4, 5 , Qiao-Yu Zhou 1, 2, 3, 4, 5 , Fang-Ya Mu 1, 2, 3, 4, 5 , Zi-Han Chen 1, 2, 3, 4, 5 , Xu-Yin Ding 1, 2, 3, 4, 5 , Min Zhang 1, 2, 3, 4, 5 , Guoyue Shi 1, 2, 3, 4, 5
Analyst ( IF 3.6 ) Pub Date : 2017-08-04 00:00:00 , DOI: 10.1039/c7an01099k Shishi Ma 1, 2, 3, 4, 5 , Qiao-Yu Zhou 1, 2, 3, 4, 5 , Fang-Ya Mu 1, 2, 3, 4, 5 , Zi-Han Chen 1, 2, 3, 4, 5 , Xu-Yin Ding 1, 2, 3, 4, 5 , Min Zhang 1, 2, 3, 4, 5 , Guoyue Shi 1, 2, 3, 4, 5
Affiliation
A novel and facile ratiometric fluorescence method for evaluating Cu2+ has been developed based on coumarin-labeled single-stranded DNA (C-ssDNA) coupled with the Cu2+-induced oxidation of o-phenylenediamine (OPD). By combining the microdialysis technique, the ratiometric fluorescence method has also been successfully exploited to monitor the cerebral Cu2+ in the rat brain, promising new opportunities for studying the cerebral Cu2+-associated physiological and pathological events.
中文翻译:
比率荧光监测脑的Cu 2+基于加上铜香豆素标记的DNA 2+的诱导氧化ö苯二胺
基于香豆素标记的单链DNA(C-ssDNA)结合Cu 2+诱导的邻苯二胺(OPD)的氧化,开发了一种新颖且简便的比率荧光分析法来评估Cu 2+。通过结合微透析技术,比率荧光法已经成功地用于监测大鼠脑中的脑Cu 2+,为研究脑Cu 2+相关的生理和病理事件提供了新的机会。
更新日期:2017-09-08
中文翻译:
比率荧光监测脑的Cu 2+基于加上铜香豆素标记的DNA 2+的诱导氧化ö苯二胺
基于香豆素标记的单链DNA(C-ssDNA)结合Cu 2+诱导的邻苯二胺(OPD)的氧化,开发了一种新颖且简便的比率荧光分析法来评估Cu 2+。通过结合微透析技术,比率荧光法已经成功地用于监测大鼠脑中的脑Cu 2+,为研究脑Cu 2+相关的生理和病理事件提供了新的机会。