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A surface acoustic wave biosensor synergizing DNA-mediated in situ silver nanoparticle growth for a highly specific and signal-amplified nucleic acid assay
Analyst ( IF 3.6 ) Pub Date : 2017-08-04 00:00:00 , DOI: 10.1039/c7an00988g Yulin Zhang 1, 2, 3, 4 , Fan Yang 1, 2, 3, 4 , Zhongyue Sun 1, 2, 3, 4 , Yu-Tao Li 1, 2, 3, 4 , Guo-Jun Zhang 1, 2, 3, 4
Analyst ( IF 3.6 ) Pub Date : 2017-08-04 00:00:00 , DOI: 10.1039/c7an00988g Yulin Zhang 1, 2, 3, 4 , Fan Yang 1, 2, 3, 4 , Zhongyue Sun 1, 2, 3, 4 , Yu-Tao Li 1, 2, 3, 4 , Guo-Jun Zhang 1, 2, 3, 4
Affiliation
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This work reports a surface acoustic wave (SAW) DNA sensor that synergizes the surface mass effect for signal-amplified and sequence-specific DNA detection in blood serum. By combining an enzyme-mediated DNA extension reaction (both viscoelastic and mass fractions) with the in situ synthesis of silver nanoparticles (mass fraction), a highly sensitive SAW biosensing interface with synergistic mass loading was tailor-engineered. As target DNA hybridized with the surface-confined capture probes, the exposed 3′-OH terminal of the target sequence could be triggered to elongate in the presence of terminal deoxynucleoside transferase (TdT) and deoxy-ribonucleoside triphosphate (dNTP), thereby producing an evident mass effect. Importantly, the extended domain can serve as a template to specifically hybridize with Ag+-binding sequences. In the presence of reducing agents, the accumulated silver ions would nucleate for the in situ synthesis of silver nanoparticles, further enhancing the mass loading. By using this approach, we observed a rapid growth event of silver nanoparticles for signal enhancement, which improved the detection limit (0.8 pM) of the SAW sensor by 3 orders of magnitude as compared to the strategy without signal amplification (at the nanomolar level). The sensor also achieved a high specificity in discriminating even a single-mismatched DNA sequence, and meanwhile could probe the low-abundance DNA molecules directly in human serum with minimal interference. These advantages make the SAW biosensor promising for practical applications, such as monitoring of molecular interactions and disease diagnostics.
中文翻译：
一种表面声波生物传感器，可协同DNA介导的原位银纳米颗粒生长，用于高度特异性和信号放大的核酸测定
这项工作报告了表面声波（SAW）DNA传感器，该传感器可协同表面质量效应，用于血清中信号放大和序列特异性DNA检测。通过将酶介导的DNA延伸反应（粘弹性和质量分数）与银纳米颗粒的原位合成（质量分数）结合起来，量身定制了具有协同质量负载的高度灵敏的SAW生物传感界面。当目标DNA与表面受限捕获探针杂交时，在存在末端脱氧核苷转移酶（TdT）和脱氧核糖核苷三磷酸（dNTP）的情况下，可以触发靶序列暴露的3'-OH末端延长。明显的质量效应。重要的是，扩展域可以用作模板，与Ag特异性杂交+-绑定序列。在还原剂的存在下，积累的银离子将在原位成核纳米银的合成，进一步提高了质量负荷。通过使用这种方法，我们观察到了用于信号增强的纳米银颗粒的快速生长事件，与没有信号放大的策略（在纳摩尔水平）相比，声表面波传感器的检测极限（0.8 pM）提高了3个数量级。 。该传感器甚至在识别单个错配的DNA序列时也具有很高的特异性，同时可以以最小的干扰直接在人血清中探测低丰度DNA分子。这些优势使声表面波生物传感器在实际应用中很有希望，例如分子相互作用的监测和疾病诊断。
更新日期：2017-09-08
中文翻译：
一种表面声波生物传感器，可协同DNA介导的原位银纳米颗粒生长，用于高度特异性和信号放大的核酸测定
这项工作报告了表面声波（SAW）DNA传感器，该传感器可协同表面质量效应，用于血清中信号放大和序列特异性DNA检测。通过将酶介导的DNA延伸反应（粘弹性和质量分数）与银纳米颗粒的原位合成（质量分数）结合起来，量身定制了具有协同质量负载的高度灵敏的SAW生物传感界面。当目标DNA与表面受限捕获探针杂交时，在存在末端脱氧核苷转移酶（TdT）和脱氧核糖核苷三磷酸（dNTP）的情况下，可以触发靶序列暴露的3'-OH末端延长。明显的质量效应。重要的是，扩展域可以用作模板，与Ag特异性杂交+-绑定序列。在还原剂的存在下，积累的银离子将在原位成核纳米银的合成，进一步提高了质量负荷。通过使用这种方法，我们观察到了用于信号增强的纳米银颗粒的快速生长事件，与没有信号放大的策略（在纳摩尔水平）相比，声表面波传感器的检测极限（0.8 pM）提高了3个数量级。 。该传感器甚至在识别单个错配的DNA序列时也具有很高的特异性，同时可以以最小的干扰直接在人血清中探测低丰度DNA分子。这些优势使声表面波生物传感器在实际应用中很有希望，例如分子相互作用的监测和疾病诊断。
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