AbstractThe authors describe a new kind of enzymatic glucose assay. The strategy involves three processes: (a) Generation of H2O2 via glucose oxidase catalyzed oxidation of glucose; (b) production of 2,3-diaminophenazine (DAP) from H2O2 and o-phenylenediamine (OPD) via peroxidase-catalyzed oxidation; and (c) a reduction of the blue fluorescence of SiNPs with emission maxima at 445 nm via an inner filter effect that is caused by DAP which is yellow and has an absorption peak at 450 nm. Fluorescence drops with increasing glucose concentrations in the 0.01 to 7 mM concentration range, and the detection limit is 3.5 μM. The assay was successfully applied to the analysis of glucose in human serum. Graphical abstractGlucose oxidase (Gox) catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide (H2O2), which then oxidizes o-phenylenediamine (OPD) to 2,3-diaminophenazine (DAP). DAP induces the fluorescence reduction of SiNPs synthesized by hydrothermal method due to inner filter effect (IFE).

中文翻译：

---

使用荧光硅纳米颗粒和过氧化物酶诱导的邻苯二胺被过氧化氢氧化引起的内部过滤器测定葡萄糖

摘要作者描述了一种新的酶促葡萄糖检测方法。该策略涉及三个过程： (a) 通过葡萄糖氧化酶催化葡萄糖氧化产生 H2O2；(b) 通过过氧化物酶催化氧化从 H2O2 和邻苯二胺 (OPD) 生产 2,3-二氨基吩嗪 (DAP)；(c) 通过内部过滤效应减少 SiNPs 的蓝色荧光，其最大发射波长为 445 nm，DAP 是黄色的，在 450 nm 处具有吸收峰。荧光在 0.01 至 7 mM 浓度范围内随着葡萄糖浓度的增加而下降，检测限为 3.5 μM。该测定已成功应用于人血清中葡萄糖的分析。图形摘要葡萄糖氧化酶（Gox）催化葡萄糖氧化为葡萄糖酸和过氧化氢（H2O2），然后将邻苯二胺 (OPD) 氧化为 2,3-二氨基吩嗪 (DAP)。由于内过滤效应 (IFE)，DAP 诱导通过水热法合成的 SiNPs 的荧光减少。