The pyrrolysine tRNA synthetase–tRNA pair can genetically encode many noncanonical amino acids through amber stop-codon suppression. This Minireview summarises the development of pyrrolysine amber stop-codon suppression, presents some of the requirements for using this technique to its greatest potential, and showcases the creative ways in which this technique has led to a better understanding of biological systems.

中文翻译：

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吡咯赖氨酸琥珀终止密码子抑制：开发和应用。

吡咯赖氨酸tRNA合成酶–tRNA对可通过琥珀色终止密码子抑制来遗传编码许多非经典氨基酸。这份Minireview总结了吡咯赖氨酸琥珀终止密码子抑制技术的发展，提出了使用该技术发挥其最大潜力的一些要求，并展示了该技术有助于更好地理解生物系统的创新方式。