Ciprofloxacin (CIP) is an antibiotic drug frequently detected in manure compost and is difficult to decompose at high temperatures, resulting in a potential threat to the environment. Microbial degradation is an effective and environmentally friendly method to degrade CIP. In this study, a thermophilic bacterium that can degrade CIP was isolated from sludge sampled from an antibiotics pharmaceutical factory. This strain is closely related to Thermus thermophilus based on 16S rRNA gene sequence analysis and is designated C419. The optimal temperature and pH values for CIP degradation are 70 °C and 6.5, respectively, and an appropriate sodium acetate concentration promotes CIP degradation. Seven major biodegradation metabolites were identified by an ultra-performance liquid chromatography tandem mass spectrometry analysis. In addition, strain C419 degraded other fluoroquinolones, including ofloxacin, norfloxacin and enrofloxacin. The supernatant from the C419 culture grown in fluoroquinolone-containing media showed attenuated antibacterial activity. These results indicate that strain C419 might be a new auxiliary bacterial resource for the biodegradation of fluoroquinolone residue in thermal environments.

中文翻译：

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Thermus sp。对环丙沙星生物降解的研究。从制药污泥中分离

环丙沙星（CIP）是一种经常在粪肥堆肥中检测到的抗生素药物，在高温下难以分解，对环境造成潜在威胁。微生物降解是降解CIP的有效且环保的方法。在这项研究中，从抗生素制药厂采样的污泥中分离出可以降解CIP的嗜热细菌。该菌株与嗜热栖热菌密切相关根据16S rRNA基因序列分析，命名为C419。CIP降解的最佳温度和pH值分别为70°C和6.5，适当的乙酸钠浓度可促进CIP降解。通过超高效液相色谱串联质谱分析确定了七种主要的生物降解代谢物。此外，菌株C419降解了其他氟喹诺酮类药物，包括氧氟沙星，诺氟沙星和恩氟沙星。在含氟喹诺酮的培养基中生长的C419培养物的上清液显示出减弱的抗菌活性。这些结果表明菌株C419可能是热环境中氟喹诺酮残留物生物降解的新辅助细菌资源。