当前位置: X-MOL 学术Anal. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
In Vivo Ambient Serotonin Measurements at Carbon-Fiber Microelectrodes
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-07 00:00:00 , DOI: 10.1021/acs.analchem.7b01257
Aya Abdalla 1 , Christopher W. Atcherley 2 , Pavithra Pathirathna 1 , Srimal Samaranayake 1 , Beidi Qiang 3 , Edsel Peña 3 , Stephen L. Morgan 1 , Michael L. Heien 4 , Parastoo Hashemi 1
Affiliation  

The mechanisms that control extracellular serotonin levels in vivo are not well-defined. This shortcoming makes it very challenging to diagnose and treat the many psychiatric disorders in which serotonin is implicated. Fast-scan cyclic voltammetry (FSCV) can measure rapid serotonin release and reuptake events but cannot report critically important ambient serotonin levels. In this Article, we use fast-scan controlled adsorption voltammetry (FSCAV), to measure serotonin’s steady-state, extracellular chemistry. We characterize the “Jackson” voltammetric waveform for FSCAV and show highly stable, selective, and sensitive ambient serotonin measurements in vitro. In vivo, we report basal serotonin levels in the CA2 region of the hippocampus as 64.9 ± 2.3 nM (n = 15 mice, weighted average ± standard error). We electrochemically and pharmacologically verify the selectivity of the serotonin signal. Finally, we develop a statistical model that incorporates the uncertainty in in vivo measurements, in addition to electrode variability, to more critically analyze the time course of pharmacological data. Our novel method is a uniquely powerful analysis tool that can provide deeper insights into the mechanisms that control serotonin’s extracellular levels.

中文翻译:

碳纤维微电极的体内环境血清素测量

体内控制细胞外血清素水平的机制尚不清楚。该缺点使得诊断和治疗与5-羟色胺有关的许多精神疾病非常具有挑战性。快速扫描循环伏安法(FSCV)可以测量5-羟色胺的快速释放和再摄取事件,但不能报告至关重要的环境5-羟色胺水平。在本文中,我们使用快速扫描控制吸附伏安法(FSCAV)来测量5-羟色胺的稳态细胞外化学。我们表征了FSCAV的“杰克逊”伏安波形,并在体外显示了高度稳定,选择性和灵敏的环境5-羟色胺测量值。在体内,我们报告海马CA2区的基础血清素水平为64.9±2.3 nM(n= 15只小鼠,加权平均值±标准误差)。我们通过电化学和药理学验证了5-羟色胺信号的选择性。最后,我们开发了一种统计模型,除了电极可变性外,该模型还结合了体内测量的不确定性,可以更严格地分析药理学数据的时程。我们新颖的方法是一种功能强大的独特分析工具,可以提供更深入的了解来控制5-羟色胺细胞外水平的机制。
更新日期:2017-09-07
down
wechat
bug