Cisplatin is a widely used anti-cancer drug, but its effect is often limited by acquired resistance to the compound during treatment. Here, we use a combination of transmission electron microscopy (TEM) and nanoscale-secondary ion mass spectrometry (NanoSIMS) to reveal differences between cisplatin uptake in human ovarian cancers cells, which are known to be susceptible to acquired resistance to cisplatin. Both cisplatin sensitive and resistant cell lines were studied, revealing markedly less cisplatin in the resistant cell line. In cisplatin sensitive cells, Pt was seen to distribute diffusely in the cells with hotspots in the nucleolus, mitochondria, and autophagosomes. Inductively coupled plasma mass spectrometry (ICP-MS) was used to validate the NanoSIMS results

中文翻译：

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敏感性/耐药性卵巢癌细胞中顺铂分布的差异：TEM / NanoSIMS研究

顺铂是一种广泛使用的抗癌药，但其作用通常受到治疗期间对该化合物的耐药性的限制。在这里，我们结合使用透射电子显微镜（TEM）和纳米级二次离子质谱（NanoSIMS）来揭示人卵巢癌细胞中顺铂吸收之间的差异，众所周知，顺铂吸收容易受到顺铂耐药性的影响。对顺铂敏感和耐药细胞系均进行了研究，发现耐药细胞系中的顺铂明显减少。在顺铂敏感细胞中，Pt在核仁，线粒体和自噬体中具有热点的细胞中散布分布。电感耦合等离子体质谱（ICP-MS）用于验证NanoSIMS结果