Enhanced Osteogenic Commitment of Human Mesenchymal Stem Cells on Polyethylene Glycol-Based Cryogel with Graphene Oxide Substrate,ACS Biomaterials Science & Engineering

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Enhanced Osteogenic Commitment of Human Mesenchymal Stem Cells on Polyethylene Glycol-Based Cryogel with Graphene Oxide Substrate
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2017-09-07 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00299
Hwan D. Kim ₁ , Jiyong Kim ₁ , Rachel H. Koh ₁ , Jimin Shim ₁ , Jong-Chan Lee ₁ , Tae-Il Kim ₂ , Nathaniel S. Hwang _{1,

3}

Affiliation

Graphene oxide (GO) is considered a comparatively recent biomaterial with enormous potential because of its nontoxicity, high dispersity, and enhanced interaction with biomolecules. These characteristics of GO can promote the interactions between the substrates and cell surfaces. In this study, we incorporated GO in a cryogel-based scaffold system to observe their influence on the osteogenic responses of human tonsil-derived mesenchymal stem cells (hTMSCs). Compared to polyethylene glycol (PEG)-based cryogel scaffold, GO-embedded PEG-based (PEGDA-GO) cryogels not only showed improved cell attachment and focal adhesion kinase (FAK) signaling activation but also enhanced cell viability. Taken together, we demonstrated that PEGDA-GO cryogels can stimulate osteogenic differentiation under an osteoinductive condition and enhance osteogenic phenotypes compared to the control group. In summary, we demonstrate that GO embedded in cryogels system is an effective biofunctionalizing scaffold to control osteogenic commitment of stem cells.

中文翻译：

氧化石墨烯基质在基于乙二醇的低温凝胶上增强人间充质干细胞的成骨作用

氧化石墨烯（GO）因其无毒，高分散性和增强的与生物分子的相互作用而被认为是一种具有巨大潜力的相对较新的生物材料。GO的这些特性可以促进底物和细胞表面之间的相互作用。在这项研究中，我们将GO纳入基于冷冻凝胶的支架系统中，以观察其对人扁桃体来源的间充质干细胞（hTMSCs）的成骨反应的影响。与基于聚乙二醇（PEG）的冷冻凝胶支架相比，GO嵌入式基于PEG的（PEGDA-GO）冷冻凝胶不仅显示出改善的细胞附着和粘着斑激酶（FAK）信号激活，而且还增强了细胞活力。在一起我们证明了与对照组相比，PEGDA-GO冷冻凝胶可以在成骨条件下刺激成骨分化并增强成骨表型。总而言之，我们证明GO嵌入冷冻系统中是一种有效的生物功能化支架，可以控制干细胞的成骨作用。

更新日期：2017-09-07

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