A pyrene based chemosensor was designed and synthesized. The pyrene fluorophore was connected with a pyridine unit through a Schiff base structure to give the sensor (L). L was tested with a variety of metal ions and exhibited high colorimetric selectivities for Cu²⁺ and Fe³⁺ over other ions. Upon binding with Cu²⁺ or Fe³⁺, L showed an obvious optical color change from colorless to pink for Cu²⁺ or orange for Fe³⁺ over a wide pH range from 3 to 12. Moreover, the fluorescence of L at 370 nm decreased sharply after bonding with Fe³⁺, while other metal ions including Cu²⁺ had no apparent interference. Thus, using such single chemosensor, Cu²⁺ and Fe³⁺ can be detected independently with high selectivity and sensitivity. The limits of detection toward Cu²⁺ and Fe³⁺ were 8.5 and 2.0 μM, respectively. DFT calculation results also proved the formation of stable coordination complexes and the phenomenon of fluorescence quenching by Fe³⁺. Furthermore, L was also successfully used as a bioimaging reagent for detection of Fe³⁺ in living cells.

设计并合成了基于based的化学传感器。through荧光团通过席夫碱结构与吡啶单元连接以得到传感器（L）。用多种金属离子对L进行了测试，与其他离子相比，L对Cu ²⁺和Fe ³⁺具有较高的比色选择性。在与铜结合²⁺或Fe ³⁺，大号显示出明显的光学颜色从无色到粉红色的对Cu变化²⁺或橙色的Fe ³⁺在宽的pH范围为3至12。此外，荧光大号在370与Fe ³⁺结合后，nm急剧下降，而包括Cu ²⁺在内的其他金属离子则没有明显的干扰。因此，使用这种单一化学传感器，可以以高选择性和灵敏度独立地检测Cu ²⁺和Fe ³⁺。对Cu ²⁺和Fe ³⁺的检出限分别为8.5和2.0μM。DFT计算结果还证明了形成稳定的配位配合物以及Fe ³⁺引起的荧光猝灭现象。此外，L还成功地用作了生物成像试剂，用于检测活细胞中的Fe ³⁺。