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Ionic Strength Sensing in Living Cells
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2017-09-06 00:00:00 , DOI: 10.1021/acschembio.7b00348
Boqun Liu 1 , Bert Poolman 1 , Arnold J. Boersma 1
Affiliation  

Knowledge of the ionic strength in cells is required to understand the in vivo biochemistry of the charged biomacromolecules. Here, we present the first sensors to determine the ionic strength in living cells, by designing protein probes based on Förster resonance energy transfer (FRET). These probes allow observation of spatiotemporal changes in the ionic strength on the single-cell level.

中文翻译:

活细胞中的离子强度传感

需要了解细胞中的离子强度才能了解带电生物大分子的体内生物化学。在这里,我们介绍了第一款通过基于Förster共振能量转移(FRET)设计蛋白质探针来确定活细胞中离子强度的传感器。这些探针可以观察单细胞水平上离子强度的时空变化。
更新日期:2017-09-07
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