Bioinstructive Coatings for Hematopoietic Stem Cell Expansion Based on Chemical Vapor Deposition Copolymerization,Biomacromolecules

当前位置： X-MOL 学术 › Biomacromolecules › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Bioinstructive Coatings for Hematopoietic Stem Cell Expansion Based on Chemical Vapor Deposition Copolymerization
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-09-06 00:00:00 , DOI: 10.1021/acs.biomac.7b00743
Anna-Lena Winkler , Meike Koenig , Alexander Welle , Vanessa Trouillet , Domenic Kratzer , Christoph Hussal , Joerg Lahann ₁ , Cornelia Lee-Thedieck

Affiliation

We report the chemical vapor deposition (CVD) of dual-functional polymer films for the specific and orthogonal immobilization of two biomolecules (notch ligand delta-like 1 (DLL1) and an RGD-peptide) that govern the fate of hematopoietic stem and progenitor cells. The composition of the CVD polymer and thus the biomolecule ratio can be tailored to investigate and optimize the influence of the relative surface concentrations of biomolecules on stem cell behavior. Prior to cell experiments, all surfaces were characterized by infrared reflection adsorption spectroscopy, time-of-flight secondary ion mass spectrometry, and X-ray photoelectron spectroscopy to confirm the presence of both biomolecules. In a proof-of-principle stem cell culture study, we show that all polymer surfaces are cytocompatible and that the proliferation of the hematopoietic stem and progenitor cells is predominantly influenced by the surface concentration of immobilized DLL1.

中文翻译：

基于化学气相沉积共聚合的造血干细胞扩增生物指导性涂层

我们报道了控制功能的两个生物分子（缺口配体δ样1（DLL1）和RGD肽）的特异性和正交固定化的双功能聚合物膜的化学气相沉积（CVD），这两个分子控制着造血干细胞和祖细胞的命运。。可以调整CVD聚合物的成分，从而调整生物分子的比例，以研究和优化生物分子的相对表面浓度对干细胞行为的影响。在进行细胞实验之前，通过红外反射吸收光谱，飞行时间二次离子质谱和X射线光电子能谱对所有表面进行表征，以确认两种生物分子的存在。在原理证明干细胞培养研究中，

更新日期：2017-09-06

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11