Boolean Logic Tree of Label-Free Dual-Signal Electrochemical Aptasensor System for Biosensing, Three-State Logic Computation, and Keypad Lock Security Operation,Analytical Chemistry

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Boolean Logic Tree of Label-Free Dual-Signal Electrochemical Aptasensor System for Biosensing, Three-State Logic Computation, and Keypad Lock Security Operation
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-06 00:00:00 , DOI: 10.1021/acs.analchem.7b01498
Jiao Yang Lu ₁ , Xin Xing Zhang ₁ , Wei Tao Huang ₁ , Qiu Yan Zhu ₁ , Xue Zhi Ding ₁ , Li Qiu Xia ₁ , Hong Qun Luo ₂ , Nian Bing Li ₂

Affiliation

The most serious and yet unsolved problems of molecular logic computing consist in how to connect molecular events in complex systems into a usable device with specific functions and how to selectively control branchy logic processes from the cascading logic systems. This report demonstrates that a Boolean logic tree is utilized to organize and connect “plug and play” chemical events DNA, nanomaterials, organic dye, biomolecule, and denaturant for developing the dual-signal electrochemical evolution aptasensor system with good resettability for amplification detection of thrombin, controllable and selectable three-state logic computation, and keypad lock security operation. The aptasensor system combines the merits of DNA-functionalized nanoamplification architecture and simple dual-signal electroactive dye brilliant cresyl blue for sensitive and selective detection of thrombin with a wide linear response range of 0.02–100 nM and a detection limit of 1.92 pM. By using these aforementioned chemical events as inputs and the differential pulse voltammetry current changes at different voltages as dual outputs, a resettable three-input biomolecular keypad lock based on sequential logic is established. Moreover, the first example of controllable and selectable three-state molecular logic computation with active-high and active-low logic functions can be implemented and allows the output ports to assume a high impediment or nothing (Z) state in addition to the 0 and 1 logic levels, effectively controlling subsequent branchy logic computation processes. Our approach is helpful in developing the advanced controllable and selectable logic computing and sensing system in large-scale integration circuits for application in biomedical engineering, intelligent sensing, and control.

中文翻译：

用于生物传感，三态逻辑计算和键盘锁安全操作的无标签双信号电化学适应传感器系统的布尔逻辑树

分子逻辑计算最严重但尚未解决的问题在于如何将复杂系统中的分子事件连接到具有特定功能的可用设备中，以及如何从级联逻辑系统中选择性地控制分支逻辑过程。该报告表明，布尔逻辑树可用于组织和连接“即插即用”化学事件，DNA，纳米材料，有机染料，生物分子和变性剂，从而开发出双信号电化学进化适体传感器系统，该系统具有良好的可重置性，可用于凝血酶的扩增检测。，可控制和可选的三态逻辑计算以及键盘锁安全操作。aptasensor系统结合了DNA功能化的纳米扩增体系结构和简单的双信号电活性染料亮丽的甲酚蓝的优点，可对凝血酶进行灵敏和选择性的检测，线性响应范围为0.02–100 nM，检测极限为1.92 pM。通过将这些上述化学事件用作输入，并将差分脉冲伏安法在不同电压下的电流变化作为双输出，建立了基于顺序逻辑的可重置三输入生物分子键盘锁。此外，可以实现具有高电平有效和低电平有效逻辑功能的可控制和可选的三态分子逻辑计算的第一个示例，该示例使输出端口除0和0之外还呈现高阻态或无（Z）状态。 1个逻辑电平，有效地控制后续的分支逻辑计算过程。我们的方法有助于在大规模集成电路中开发先进的可控和可选逻辑计算和传感系统，以应用于生物医学工程，智能传感和控制。

更新日期：2017-09-06

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