Applications of vibrational tags in biological imaging by Raman microscopy,Analyst

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Applications of vibrational tags in biological imaging by Raman microscopy
Analyst ( IF 3.6 ) Pub Date : 2017-08-15 00:00:00 , DOI: 10.1039/c7an01001j
Zhilun Zhao _{1,

2,

3,

4} , Yihui Shen _{1,

2,

3,

4} , Fanghao Hu _{1,

2,

3,

4} , Wei Min _{1,

2,

3,

4,

5}

Affiliation

As a superb tool to visualize and study the spatial-temporal distribution of chemicals, Raman microscopy has made a big impact in many disciplines of science. While label-free imaging has been the prevailing strategy in Raman microscopy, recent development and applications of vibrational/Raman tags, particularly when coupled with stimulated Raman scattering (SRS) microscopy, have generated intense excitement in biomedical imaging. SRS imaging of vibrational tags has enabled researchers to study a wide range of small biomolecules with high specificity, sensitivity and multiplex capability, at a single live cell level, tissue level or even in vivo. As reviewed in this article, this platform has facilitated imaging distribution and dynamics of small molecules such as glucose, lipids, amino acids, nucleic acids, and drugs that are otherwise difficult to monitor with other means. As both the vibrational tags and Raman instrumental development progress rapidly and synergistically, we anticipate that this technique will shed light onto an even broader spectrum of biomedical problems.

中文翻译：

振动标签在拉曼显微镜生物成像中的应用

作为可视化和研究化学物质时空分布的绝佳工具，拉曼显微镜在许多科学领域都产生了巨大影响。尽管无标记成像一直是拉曼显微镜中的主要策略，但振动/拉曼标签的最新开发和应用，特别是与受激拉曼散射（SRS）显微镜结合使用时，在生物医学成像中引起了极大的兴奋。SRS振动标签成像使研究人员能够在单个活细胞水平，组织水平甚至在体内研究具有高特异性，灵敏度和多重能力的各种小型生物分子。如本文所述，该平台促进了葡萄糖，脂质，氨基酸，核酸和药物等小分子的成像分布和动力学，而这些小分子很难用其他手段进行监测。随着振动标签和拉曼仪器的发展迅速而协同地发展，我们预计该技术将为更广泛的生物医学问题提供启示。

更新日期：2017-09-06

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