Mycotoxins occur naturally as agricultural contaminants all over the world. The toxic effects of some of their metabolites are known and their presence regulated in food and feed. This paper describes two methods for the detection of toxins of type-A trichothecenes group, and their modified forms, using mass spectrometry. Ultra-performance liquid chromatography coupled to mass spectrometry-ion trap-time of flight (UPLC-MS-IT-TOF) was employed to characterize the fragmentation pathways of 10 type-A trichothecenes, and characteristic ions were tentatively identified in scan mode through their accurate masses. Unknown signals were detected in a F. sporotrichioides extract, which afterwards were identified as seven modified forms of neosolaniol (NEO) and T-2 toxin. Then, UPLC coupled to tandem mass spectrometry (MS/MS) was employed to develop a precursor ion scanning method that can be used as a screening tool to detect any modified type-A trichothecenes.

霉菌毒素是世界范围内的自然农业污染物。某些代谢物的毒性作用是已知的，它们在食物和饲料中的存在也受到调节​​。本文介绍了两种使用质谱法检测A型毛发纤毛虫基团毒素的方法及其修饰形式。采用超高效液相色谱-质谱联用-离子阱飞行时间（UPLC-MS-IT-TOF）表征了10种A型三氯噻吩的碎裂途径，并通过扫描模式初步鉴定了特征离子准确的质量。在孢子虫中检测到未知信号提取物，其后被鉴定为七种新形式的新松香酚（NEO）和T-2毒素。然后，将UPLC与串联质谱（MS / MS）耦合，以开发前体离子扫描方法，该方法可用作筛选工具，以检测任何修饰的A型三色六肽。