This review gives an updated overview on keratinocyte transplantation in burn wounds concentrating on application methods and future therapeutic cell delivery options with a special interest in hydrogels and spray devices for cell delivery.

To achieve faster re-epithelialisation of burn wounds, the original autologous keratinocyte culture and transplantation technique was introduced over 3 decades ago. Application types of keratinocytes transplantation have improved from cell sheets to single-cell solutions delivered with a spray system. However, further enhancement of cell culture, cell viability and function in vivo, cell carrier and cell delivery systems remain themes of interest.

Hydrogels such as chitosan, alginate, fibrin and collagen are frequently used in burn wound care and have advantageous characteristics as cell carriers.

Future approaches of keratinocyte transplantation involve spray devices, but optimisation of application technique and carrier type is necessary.

本综述对烧伤创面角质形成细胞移植进行了最新概述，重点关注应用方法和未来的治疗性细胞递送选择，特别关注用于细胞递送的水凝胶和喷雾装置。

为了实现烧伤创面更快的上皮再生，原始的自体角质形成细胞培养和移植技术在三十多年前就被引入。角质形成细胞移植的应用类型已从细胞片改进为通过喷雾系统输送的单细胞溶液。然而，进一步增强细胞培养、细胞活力和体内功能、细胞载体和细胞递送系统仍然是人们感兴趣的主题。

壳聚糖、藻酸盐、纤维蛋白和胶原蛋白等水凝胶经常用于烧伤创面护理，并且具有作为细胞载体的有利特性。

未来的角质形成细胞移植方法涉及喷雾装置，但有必要优化应用技术和载体类型。