Interplay of Geometric Cues and RGD/BMP-2 Crosstalk in Directing Stem Cell Fate,ACS Biomaterials Science & Engineering

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Interplay of Geometric Cues and RGD/BMP-2 Crosstalk in Directing Stem Cell Fate
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2017-09-05 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00279
Ibrahim Bilem _{1,

2,

3,

4,

5} , Pascale Chevallier _{1,

2} , Laurent Plawinski _{3,

4,

5} , Eli D. Sone ₆ , Marie-Christine Durrieu _{3,

4,

5} , Gaétan Laroche _{1,

2}

Affiliation

Within the native microenvironment, extracellular matrix (ECM) components are thought to display a complex and heterogeneous distribution, spanning several length scales. Herein, the objective is to mimic, in vitro, the hierarchical organization of proteins and growth factors as well as their crosstalk. Photolithography technique was used to adjacently pattern geometrically defined regions of RGD and BMP-2 mimetic peptides onto glass substrates. These ECM-derived ligands are known to jointly regulate mesenchymal stem cells (MSCs) osteogenic differentiation. By manipulating the spatial distribution of dually grafted peptides, the extent of human MSCs osteogenic differentiation was significantly affected, depending on the shape of peptide micropatterns. Our data highlight the existence of a strong interplay between geometric cues and biochemical signals. Such in vitro systems provide a valuable tool to investigate mechanisms by which multiple ECM cues overlap to regulate stem cell fate, thereby contributing to the design of bioinspired biomaterials for bone tissue engineering applications.

中文翻译：

几何线索与RGD / BMP-2串扰在指导干细胞命运中的相互作用

在天然微环境中，细胞外基质（ECM）成分被认为显示出复杂且异质的分布，跨越了多个长度尺度。在此，目的是在体外模拟蛋白质和生长因子及其串扰的分级组织。使用光刻技术将RGD和BMP-2模拟肽的几何定义区域相邻地图案化到玻璃基板上。已知这些ECM衍生的配体共同调节间充质干细胞（MSC）成骨分化。通过操纵双嫁接肽的空间分布，人MSC的成骨分化程度受到显着影响，具体取决于肽微图案的形状。我们的数据突显了几何线索与生化信号之间存在强烈的相互作用。这样的体外系统提供了一种有价值的工具，可用于研究多个ECM线索重叠以调节干细胞命运的机制，从而有助于为骨组织工程应用设计生物启发性生物材料。

更新日期：2017-09-06

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