Long-Term and Programmable Bacterial Subculture in Completely Automated Microchemostats,Analytical Chemistry

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Long-Term and Programmable Bacterial Subculture in Completely Automated Microchemostats
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-05 00:00:00 , DOI: 10.1021/acs.analchem.7b01076
Minseok Kim ₁ , Juyeol Bae ₁ , Taesung Kim ₁

Affiliation

A controllable microchemostat can provide an ideal, powerful means to study the growth behavior of microorganisms by improving conventional macroscale chemostat. However, a challenge remains for implementing both continuous growth and active population control of microorganisms at the same time because they keep communicating with nearby culture environments by regulating their metabolism. Here, we present a novel microchemostat that enables reversible bacterial isolation, continuous chemical refreshment, and dynamic physicochemical stimulation. The microchemostat not only controls bacterial growth and subculture conditions in a completely automated and programmed manner but it also makes it possible to manipulate bacterial populations from a single bacterium to an ultrahigh density for long-term subculture periods with ultralow reagent consumption. Moreover, the microchemostat enables in situ measurement and feedback control of bacterial growth and population through various subculture programming modes that are sequentially performed using a single microchemostat over 720 h; to the best of our knowledge, this is the longest microchemostat culture of bacterial cells reported to date. Hence, we ensure that the microchemostat can be further applied to a wide range of microbial studies on a single chip, such as nutrient optimization, genetic induction, environmental selection, high-throughput screening, and evolutionary adaptation.

中文翻译：

全自动微化学恒温器中的长期可编程细菌亚培养

可控的微化学稳定剂可通过改进常规的大型化学恒温器提供理想，强大的方法来研究微生物的生长行为。然而，同时实现微生物的连续生长和主动种群控制仍然是一个挑战，因为它们通过调节其新陈代谢与附近的培养环境保持联系。在这里，我们介绍了一种新型的微化学稳定剂，可实现可逆的细菌分离，连续的化学更新和动态的物理化学刺激。微型化学稳压器不仅可以完全自动化和程序化的方式控制细菌的生长和继代培养条件，而且还可以在长期的继代培养期间以超低的试剂消耗量将细菌种群从单一细菌控制到超高密度。此外，微化学稳定剂能够通过各种亚培养编程模式实现对细菌生长和种群的原位测量和反馈控制，这些模式使用单个微化学稳定器在720小时内依次执行；据我们所知，这是迄今为止报道的最长的细菌细胞微化学稳定剂培养物。因此，我们确保将微化学稳定剂进一步应用于单个芯片上的各种微生物研究，例如营养优化，基因诱导，环境选择，

更新日期：2017-09-06

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