Summary In a recent paper: Goldstein I, Baek S, Presman DM, Paakinaho V, Swinstead EE, Hager GL. Transcription factor assisted loading and enhancer dynamics dictate the hepatic fasting response. Genome Res. 2017 Mar;27(3):427-39., the authors used a number of functional genomics approaches to explore the transcriptional regulatory dynamics that occur during hepatic fasting. They used chromatin landscape data to identify key fasting-related transcription factors, four of which were further investigated because they are known players of the fasting response: CEBPB (CCAAT enhancer binding-beta), CREB1 (cAMP responsive element binding protein I), GR (glucocorticoid receptor), and PPARA (peroxisome proliferator activated receptor alpha). The authors described two operating modules (GR-CREB1 and PPARA-GR) with synergistic effects driving gluconeogenesis through an assisted loading model and fatty acid oxidation/ketogenesis through a transcription factor cascade, respectively. Finally, using single-cell tracking, they confirmed that GR facilitates CREB1 binding to DNA. This article is protected by copyright. All rights reserved.

中文翻译：

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绘制禁食期间小鼠肝脏转录调控变化的图表


摘要 在最近的一篇论文中：Goldstein I、Baek S、Presman DM、Paakinaho V、Swinstead EE、Hager GL。转录因子辅助加载和增强子动力学决定了肝脏的禁食反应。基因组研究。 2017年3月；27(3):427-39.，作者使用了多种功能基因组学方法来探索肝脏禁食期间发生的转录调控动态。他们使用染色质景观数据来识别与禁食相关的关键转录因子，其中四个因是禁食反应的已知参与者而被进一步研究：CEBPB（CCAAT 增强子结合-β）、CREB1（cAMP 反应元件结合蛋白 I）、GR （糖皮质激素受体）和 PPARA（过氧化物酶体增殖物激活受体 α）。作者描述了两个具有协同效应的操作模块（GR-CREB1 和 PPARA-GR），分别通过辅助加载模型驱动糖异生，并通过转录因子级联驱动脂肪酸氧化/酮生成。最后，利用单细胞追踪，他们证实 GR 促进 CREB1 与 DNA 结合。本文受版权保护。版权所有。