α-Amanitin is the main lethal component of amanita mushrooms, and data on its toxicokinetics are few. The aim of this study was to develop a sensitive and cost-effective method to identify α-amanitin and investigate its toxicokinetic parameters using liquid chromatography-triple quadrupole tandem mass spectrometry. The colchicine was used as the internal standard (IS). The compounds were extracted from plasma samples by protein precipitation with acetonitrile (containing 1% formic acid). The analysis was performed through multiple reactions monitoring. The molecular ions and fragment ions of α-amanitin could be used as characteristic ions to perform qualitative analysis of α-amanitin. The assay was successfully validated by selectivity, linearity, matrix effect, precision and accuracy, recovery and stability according to the U.S. Food and Drug Administration Guidance, and applied to study the toxicokinetic profile of α-amanitin in rats after a single intraperitoneal administration.

α-天冬氨酸是伞形毒蕈的主要致死成分，其毒代动力学的数据很少。这项研究的目的是开发一种灵敏且经济高效的方法来鉴定α-amanitin，并使用液相色谱-三重串联四极杆串联质谱法研究其毒物动力学参数。秋水仙碱被用作内标（IS）。通过用乙腈（含1％甲酸）进行蛋白质沉淀从血浆样品中提取化合物。通过多反应监测进行分析。α-amanitin的分子离子和碎片离子可以作为特征离子对α-amanitin进行定性分析。根据美国标准，该方法已通过选择性，线性，基质效应，精密度和准确性，回收率和稳定性得到了成功验证