Covalent organic frameworks (COFs), as an emerging class of crystalline porous organic polymers, have great potential for applications in chromatographic separation owning to their fascinating crystalline structures and outstanding properties. However, development of COF materials as novel stationary phases in high performance liquid chromatography (HPLC) is just in its infancy. Herein, we report the design and construction of a new hydrazone-linked chiral COF, termed BtaMth COF, from a chiral hydrazide building block (Mth) and present a one-pot synthetic method for the fabrication of [email protected]₂ composite for HPLC separation of isomers. The as-synthesized BtaMth chiral COF displays good crystallinity, high porosity, as well as excellent chemical stability. Meanwhile, the fabricated HPLC column by using [email protected]₂ composite as the new stationary phase exhibits high resolution performances for the separation of positional isomers including nitrotoluene and nitrochlorobenzene, as well as cis-trans isomers including beta-cypermethrin and metconazole. Additionally, some effects such as the composition of the mobile phase and column temperature for HPLC separations on the [email protected]₂ packed column also have been studied in detail. The successful applications indicate the great potentials of hydrazone-linked chiral COF–silica composite as novel stationary phase for the efficient HPLC separation.

作为新兴的一类结晶多孔有机聚合物，共价有机骨架（COF）由于其迷人的晶体结构和出色的性能而在色谱分离中具有广阔的应用前景。但是，COF材料在高效液相色谱（HPLC）中作为新型固定相的开发才刚刚起步。本文中，我们报道了一种从手性酰肼结构单元（Mth）制备的新型连接手性COF（称为BtaMth COF）的设计和构建，并提出了一种制备[受电子邮件保护] ₂的单罐合成方法。用于HPLC分离异构体的复合材料。合成后的BtaMth手性COF表现出良好的结晶度，高孔隙率以及出色的化学稳定性。同时，通过使用[电子邮件保护]制造HPLC柱₂复合物作为新的固定相表现出高分辨率的演出的位置异构体，包括硝基甲苯和硝基氯苯，以及分离顺式-反式异构体，包括高效氯氰菊酯和叶菌唑。此外，在[受电子邮件保护的] ₂上还存在一些影响，例如用于HPLC分离的流动相的组成和柱温填充柱也已进行了详细研究。成功的应用表明，linked连接的手性COF-二氧化硅复合物作为高效液相色谱分离的新型固定相具有巨大的潜力。