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High-Throughput Single-Particle Analysis of Metal-Enhanced Fluorescence in Free Solution Using Ag@SiO2 Core–Shell Nanoparticles
ACS Sensors ( IF 8.2 ) Pub Date : 2017-09-05 00:00:00 , DOI: 10.1021/acssensors.7b00522 Ya Yan 1 , Lingyan Meng 1 , Wenqiang Zhang 1 , Yan Zheng 1 , Shuo Wang 1 , Bin Ren 1 , Zhilin Yang 1 , Xiaomei Yan 1
ACS Sensors ( IF 8.2 ) Pub Date : 2017-09-05 00:00:00 , DOI: 10.1021/acssensors.7b00522 Ya Yan 1 , Lingyan Meng 1 , Wenqiang Zhang 1 , Yan Zheng 1 , Shuo Wang 1 , Bin Ren 1 , Zhilin Yang 1 , Xiaomei Yan 1
Affiliation
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Metal-enhanced fluorescence (MEF) based on localized surface plasmon resonance (LSPR) is an effective strategy to increase the detection sensitivity in biotechnology and biomedicine. Because plasmonic nanoparticles are intrinsically heterogeneous, high-throughput single-particle analysis of MEF in free solution are highly demanded for the mechanistic understanding and control of this nanoscale process. Here, we report the application of a laboratory-built high-sensitivity flow cytometer (HSFCM) to investigate the fluorescence-enhancing effect of individual plasmonic nanoparticles on nearby fluorophore molecules. Ag@SiO2 core–shell nanoparticles were used as the model system which comprised a silver core, a silica shell, and an FITC-doped thin layer of silica shell. FITC-doped silica nanoparticles of the same particle size but without silver core were used as the counterparts. Both the side scattering and fluorescence signals of single nanoparticles in suspension were measured simultaneously by the HSFCM at a speed of thousands of particles per minute. The roles of silver core size (40–100 nm) and fluorophore–metal distance (5–30 nm) were systematically examined. Fluorescence enhancement factor exceeding 30 was observed at silver core size of 70 nm and silica shell thickness of 5 nm. Compared with ensemble-averaged spectrofluorometric measurements, our experimental observation at the single-particle level was well supported by the finite difference time domain (FDTD) calculation. It allows us to achieve a fundamental understanding of MEF, which is important to the design and control of plasmonic nanostructures for efficient fluorescence enhancement.
中文翻译：
Ag @ SiO 2核壳纳米粒子在自由溶液中高通量金属增强荧光的单颗粒分析
基于局部表面等离子体激元共振（LSPR）的金属增强荧光（MEF）是提高生物技术和生物医学中检测灵敏度的有效策略。由于等离激元纳米粒子本质上是异质的，因此对于这种纳米级过程的机理理解和控制，对游离溶液中MEF的高通量单粒子分析提出了很高的要求。在这里，我们报告了实验室建造的高灵敏度流式细胞仪（HSFCM）的应用，以研究单个等离子体纳米颗粒对附近的荧光团分子的荧光增强作用。银@SiO 2核壳纳米粒子被用作模型系统，包括银核，二氧化硅壳和FITC掺杂的二氧化硅壳薄层。使用相同粒径但没有银芯的FITC掺杂的二氧化硅纳米粒子作为对应物。通过HSFCM以每分钟数千个粒子的速度同时测量悬浮液中单个纳米粒子的侧面散射和荧光信号。系统检查了银核尺寸（40-100 nm）和荧光团-金属距离（5-30 nm）的作用。在70nm的银芯尺寸和5nm的二氧化硅壳厚度下观察到超过30的荧光增强因子。与整体平均荧光分光光度法测量相比，我们在单粒子水平上的实验观察得到了时差有限时域（FDTD）计算的良好支持。它使我们对MEF有了一个基本的了解，这对等离子体和纳米结构的设计和控制对于有效增强荧光很重要。
更新日期：2017-09-05
中文翻译：
Ag @ SiO 2核壳纳米粒子在自由溶液中高通量金属增强荧光的单颗粒分析
基于局部表面等离子体激元共振（LSPR）的金属增强荧光（MEF）是提高生物技术和生物医学中检测灵敏度的有效策略。由于等离激元纳米粒子本质上是异质的，因此对于这种纳米级过程的机理理解和控制，对游离溶液中MEF的高通量单粒子分析提出了很高的要求。在这里，我们报告了实验室建造的高灵敏度流式细胞仪（HSFCM）的应用，以研究单个等离子体纳米颗粒对附近的荧光团分子的荧光增强作用。银@SiO 2核壳纳米粒子被用作模型系统，包括银核，二氧化硅壳和FITC掺杂的二氧化硅壳薄层。使用相同粒径但没有银芯的FITC掺杂的二氧化硅纳米粒子作为对应物。通过HSFCM以每分钟数千个粒子的速度同时测量悬浮液中单个纳米粒子的侧面散射和荧光信号。系统检查了银核尺寸（40-100 nm）和荧光团-金属距离（5-30 nm）的作用。在70nm的银芯尺寸和5nm的二氧化硅壳厚度下观察到超过30的荧光增强因子。与整体平均荧光分光光度法测量相比，我们在单粒子水平上的实验观察得到了时差有限时域（FDTD）计算的良好支持。它使我们对MEF有了一个基本的了解，这对等离子体和纳米结构的设计和控制对于有效增强荧光很重要。
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