AbstractThe authors describe an amperometric assay for the detection of prostate specific antigen (PSA) that combines the advantages of using a molecularly imprinted polymer (MIP) and of a nanocomposite composed of graphene nanoplatelets (graphene sheets; GS), gold nanoparticles (AuNPs) and chitosan (Chit). The GS-AuNP composite was synthesized by a single-step reduction of GS and HAuCl4 solution. The MIP was synthesized by electropolymerization of dopamine and characterized by scanning electron microscopy and differential pulse voltammetry (DPV). Sensitivity is strongly improved by the magnified current obtained by using the GS-AuNP hybrid. Chit was further employed as a film-forming material to prevent the leakage of nanomaterials. Under optimized conditions, the method displays good analytical performance for the detection of PSA by DPV and by using hexacyanoferrate as the electrochemical probe. The peak current (typically measured at 0.16 V vs. SCE) increases linearly in the 1 pg mL−1 to 100 ng mL−1 PSA concentration range, and the detection limit is 0.15 pg mL−1 at a signal to noise ratio of 3. The method was successfully applied to the determination of PSA in serum. The assay is highly selective, sensitive, reproducible and stable. In our perception, it represents an attractive alternative to the commercially available ELISA kits for PSA. Graphical abstractSchematic of a molecularly imprinted material obtained via electropolymerization of dopamine onto gold nanoparticles and placed, along with graphene nanoplatelets and chitosan on an electrode to detect prostate specific antigen (PSA). The sensor shows good specificity and accuracy for PSA detection in female serum.

中文翻译：

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一种用石墨烯纳米片、金纳米颗粒和壳聚糖修饰并涂有分子印迹聚合物的玻碳电极，用于高度灵敏地测定前列腺特异性抗原

摘要作者描述了一种用于检测前列腺特异性抗原 (PSA) 的电流分析法，它结合了使用分子印迹聚合物 (MIP) 和由石墨烯纳米片（石墨烯片；GS）、金纳米颗粒 (AuNPs) 和纳米复合材料组成的纳米复合材料的优点。壳聚糖（Chit）。GS-AuNP 复合材料是通过一步还原 GS 和 HAuCl4 溶液合成的。MIP 是通过多巴胺的电聚合合成的，并通过扫描电子显微镜和差分脉冲伏安法 (DPV) 进行表征。通过使用 GS-AuNP 混合体获得的放大电流大大提高了灵敏度。Chit 被进一步用作成膜材料，以防止纳米材料的泄漏。在优化条件下，该方法对DPV和六氰基铁酸盐作为电化学探针检测PSA表现出良好的分析性能。峰值电流（通常在 0.16 V vs. SCE 下测量）在 1 pg mL-1 至 100 ng mL-1 PSA 浓度范围内线性增加，检测限为 0.15 pg mL-1，信噪比为 3 . 该方法成功应用于血清中PSA的测定。该测定具有高度选择性、灵敏性、重现性和稳定性。在我们看来，它代表了市售 PSA ELISA 试剂盒的有吸引力的替代品。图形摘要通过多巴胺在金纳米颗粒上的电聚合获得的分子印迹材料的示意图，并与石墨烯纳米血小板和壳聚糖一起放置在电极上以检测前列腺特异性抗原 (PSA)。