AbstractThe authors describe an impedimetric nanosensor for aptamer-mediated detection of ATP. A triangular junction of polysilicon substrate is used as a transducer. The aptamer was immobilized on the substrate via biotin-streptavidin interaction. ATP can be detected by this method in the 10 to 100 nM concentration range at room temperature and estimated the signal to noise ratio above the range of 3σ. It was applied to the detection of ATP in 1:1000 diluted human urine. The desired transducer is highly reproducible and specific. It is selective over closely related molecules such as guanosine triphosphate, cytidine triphosphate and uridine triphosphate. The method is perceived to represent a valuable tool for the diagnosis for infections and inflammations. The novelties shown in this study compared to other sensors are, consumption of low sample volume (~ 1 μl), non-invasive and suitable with AC and DC currents. Graphical abstractAptamer-functionalized Impedimetric Nanosensor for ATP Determination in Human Urine by a Triangular Junction Transducer.

中文翻译：

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通过使用功能化的阻抗纳米传感器和三角形结换能器介导的基于适体的 ATP 测定

摘要作者描述了一种用于适体介导的 ATP 检测的阻抗纳米传感器。多晶硅衬底的三角形结用作换能器。适体通过生物素-链霉亲和素相互作用固定在底物上。这种方法可以在室温下在 10 到 100 nM 的浓度范围内检测到 ATP，并估计信噪比高于 3σ 范围。应用于1:1000稀释人尿中ATP的检测。所需的换能器具有高度可重复性和特异性。它对密切相关的分子具有选择性，例如三磷酸鸟苷、三磷酸胞苷和三磷酸尿苷。该方法被认为是诊断感染和炎症的有价值的工具。与其他传感器相比，本研究中显示的新颖性是，消耗低样品体积 (~ 1 μl)，无创且适用于交流和直流电流。用于通过三角形结换能器测定人尿中 ATP 的图形摘要适体功能化阻抗纳米传感器。