AbstractThe authors describe a glassy carbon electrode (GCE) modified with a multiporous nanofibers prepared from SnO2, polyaniline and hemoglobin, and its application to the amperometric determination of hydrogen peroxide (H2O2). The SnO2 nanofibers were prepared first and then polymerized with polyaniline. Then, the redox protein hemoglobin was co-immobilized with the nanofibers on the surface of the GCE. Chitosan was finally used to improve the stability of the modified GCE. Direct electrochemistry of Hemoglobin at the modified electrode revealed a pair of well-defined redox peaks, with anodic and cathodic peaks at −0.15 and −0.3 V, respectively. The modified GCE exhibits high catalytic response to hydrogen peroxide. Best operated at −0.4 V (vs Ag/AgCl), it displays a linear amperometric response that covers the 2 to 160 μM H2O2 concentration range, with a lower detection limit of 0.5 μM. The biosensor has good storage stability, reproducibility and repeatability. Graphical abstractA porous electrode material is presented that consists of tin oxide modified with polyaniline and hemoglobin to achieve better electrical contact, electron transfer rates, and sensing performance. The electrode was applied to the detection of H2O2.

中文翻译：

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一种用 SnO2 纳米纤维、聚苯胺和血红蛋白修饰的玻璃碳电极，用于改进过氧化氢的电流检测

摘要作者描述了一种用 SnO2、聚苯胺和血红蛋白制备的多孔纳米纤维改性的玻璃碳电极 (GCE)，及其在过氧化氢 (H2O2) 的安培测定中的应用。首先制备SnO2纳米纤维，然后与聚苯胺聚合。然后，氧化还原蛋白血红蛋白与 GCE 表面的纳米纤维共同固定。壳聚糖最终用于提高改性GCE的稳定性。修饰电极处血红蛋白的直接电化学显示一对明确的氧化还原峰，阳极和阴极峰分别位于 -0.15 和 -0.3 V。改性 GCE 对过氧化氢表现出高催化反应。最佳工作电压为 -0.4 V（相对于 Ag/AgCl），它显示线性电流响应，涵盖 2 至 160 μM H2O2 浓度范围，检测下限为 0.5 μM。该生物传感器具有良好的储存稳定性、重现性和重复性。图形摘要提出了一种多孔电极材料，该材料由用聚苯胺和血红蛋白改性的氧化锡组成，以实现更好的电接触、电子转移率和传感性能。该电极用于检测H2O2。