In the thymus, hematopoietic progenitors commit to the T cell lineage and undergo sequential differentiation to generate diverse T cell subsets, including major histocompatibility complex (MHC)–restricted αβ T cell receptor (TCR) T cells and non–MHC-restricted γδ TCR T cells. The factors controlling precursor commitment and their subsequent maturation and specification into αβ TCR versus γδ TCR T cells remain unclear. Here, we show that the tyrosine phosphatase PTPN2 attenuates STAT5 (signal transducer and activator of transcription 5) signaling to regulate T cell lineage commitment and SRC family kinase LCK and STAT5 signaling to regulate αβ TCR versus γδ TCR T cell development. Our findings identify PTPN2 as an important regulator of critical checkpoints that dictate the commitment of multipotent precursors to the T cell lineage and their subsequent maturation into αβ TCR or γδ TCR T cells.

在胸腺中，造血祖细胞参与T细胞谱系并经历顺序分化，以产生多种T细胞亚群，包括主要组织相容性复合物（MHC）限制的αβT细胞受体（TCR）T细胞和非MHC限制的γδTCR T细胞。尚不清楚控制前体承诺以及随后成熟和确定为αβTCR与γδTCR T细胞的因素。在这里，我们显示酪氨酸磷酸酶PTPN2减弱STAT5（信号转导和转录激活因子5）信号来调节T细胞谱系定型，而SRC家族激酶LCK和STAT5信号传导则调节αβTCR对γδTCR T细胞发育。