A simple paper-based sensor with electrochemical detection was first developed for rapid, selective, and sensitive determination of acetylcholinesterase (AChE). The screen-printed graphene electrode was used as working electrode providing sensitivity for the sensor. The amperometric detection of AChE is based on the determination of thiocholine (TCh) produced from hydrolysis of acetylthiocholine chloride (ATCh) by AChE. To detect TCh, the ATCh immobilized sheet was stacked onto the detection sheet using double adhesive tape, then samples of AChE were dropped on the back side of an ATCh immobilized sheet with only 1 min of incubation time. To avoid interference, glutathione (GSH), the potential of 0.5 V vs. Ag/AgCl was applied onto a graphene electrode and the current, which depends on AChE concentration, was measured. Under optimized conditions, the limit of detection (LOD) from the experiment of AChE determination was 0.1 U/mL with AChE concentration in range of 0.1–15 U/mL. The data correlated well with the data obtained using spectrophotometric method. The developed sensor had been successfully applied to detect AChE in blood samples.

首先开发了一种具有电化学检测功能的简单纸质传感器，用于快速，选择性和灵敏地测定乙酰胆碱酯酶（AChE）。丝网印刷的石墨烯电极用作工作电极，可为传感器提供灵敏度。AChE的安培检测基于对AChE水解乙酰基硫代胆碱氯化物（ATCh）产生的硫代胆碱（TCh）的确定。为了检测TCh，使用双面胶带将固定有ATCh的薄片堆叠到检测薄片上，然后仅用1分钟的孵育时间将AChE样品滴到固定有ATCh的薄片的背面。为了避免干扰，将谷胱甘肽（GSH）施加0.5 V vs. Ag / AgCl的电势到石墨烯电极上，并测量取决于AChE浓度的电流。在优化条件下，AChE测定实验的检测限（LOD）为0.1 U / mL，AChE浓度在0.1–15 U / mL范围内。数据与使用分光光度法获得的数据具有很好的相关性。研发的传感器已成功应用于血液样本中的AChE检测。