In this article, red-emitting copper nanoclusters (Cu NCs) were prepared in the matrix of bovine serum albumin (BSA) through using N₂H₄·H₂O as a reducing agent and BSA as a stabilizer. In the aqueous suspension of BSA-Cu NCs, the addition of rutin enabled the combination of rutin with BSA around Cu NCs via hydrogen bond and electrostatic interactions between functional groups of BSA and rutin. The approaching of rutin to Cu NCs induced that the fluorescence (FL) of Cu NCs was quenched by rutin due to the electron transfer from Cu NCs to rutin. Under the optimal conditions, the FL of Cu NCs was linearly decreased with the increase of rutin concentration from 0.1 to100 μM, together with a low detection limit of ca. 0.02 μM. The FL quenching responses of Cu NCs were highly selective and sensitive towards rutin, over potential interferents in real human serum samples. Based on BSA-Cu NCs, this as-proposed FL probe exhibited a superior capability of rutin detection in real human serum samples. Significantly, this probe of rutin could realize the FL sensing of rutin and the naked-eye visual FL imaging detection of rutin in aqueous solution and on filter paper.

本文以N ₂ H ₄ ·H ₂ O为还原剂，BSA为稳定剂，在牛血清白蛋白（BSA）的基质中制备了红色发光的铜纳米簇（Cu NCs）。在BSA-Cu NCs的水悬浮液中，添加芦丁可以通过氢键和BSA与芦丁的官能团之间的静电相互作用，将芦丁与BSA结合在Cu NCs周围。芦丁接近Cu NCs导致Cu NCs的荧光（FL）被芦丁淬灭，这是由于电子从Cu NCs转移到芦丁。在最佳条件下，随着芦丁浓度从0.1μm增加到100μM，Cu NCs的FL线性降低，并且检测限较低。0.02微米 Cu NCs的FL淬灭反应对芦丁具有很高的选择性，并且对芦丁的敏感性高于实际人类血清样品中的潜在干扰物。基于BSA-Cu NCs，这种建议的FL探针在真实的人血清样品中显示出优异的芦丁检测能力。重要的是，这种芦丁探针可以实现对芦丁的FL感测和肉眼可见FL成像检测水溶液和滤纸上的芦丁。