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Ultrasensitive chemiluminescent biosensor for the detection of cholesterol based on synergetic peroxidase-like activity of MoS2 and graphene quantum dots
Talanta ( IF 5.6 ) Pub Date : 2017-09-01 , DOI: 10.1016/j.talanta.2017.08.107
Javad Hassanzadeh , Alireza Khataee

Developing a novel non-enzyme mimetic in biosensors is of great significance. Here, a synergetic peroxidase-like activity was disclosed for mixed MoS2 quantum dots (MoS2 QDs) and graphene quantum dots (GQDs). The high catalytic effect of this mixture was studied on the chemiluminescence system. It was observed that the simultaneous presence of MoS2 QDs and GQDs had a powerful enhancing effect on the chemiluminescence (CL) emission of rhodamine B (RB)-H2O2 reaction. MoS2 QDs and GQDs mixture (prepared with a ratio of 3:2) showed a superior catalytic activity when compared to each of the constituents. A linear relationship was acquired between the CL emission intensity and H2O2 concentration in the range of 1.5–460 nmol L−1. On the other hand, since the enzymatic oxidation of cholesterol leads to the production of H2O2; the offered CL system was examined to detect cholesterol after its oxidation by cholesterol oxidase (ChOx) enzyme. Herein, a further improvement was achieved by MoS2 nanosheets. The MoS2 nanosheets increased the performance of ChOx in cholesterol oxidation process. The obtained results confirmed a highly selective and sensitive determination of cholesterol concentration in a linear dynamic range of 0.08–300 µmol L−1, with a detection limit (3S) of 35 nmol L−1. The developed method was successfully applied for the detection of cholesterol level in human serum samples.



中文翻译:

基于MoS 2和石墨烯量子点的协同过氧化物酶样活性的用于胆固醇检测的超灵敏化学发光生物传感器

在生物传感器中开发新型的非酶模拟物具有重要意义。在此,公开了对于混合的MoS 2量子点(MoS 2 QDs)和石墨烯量子点(GQDs)的协同过氧化物酶活性。研究了该混合物对化学发光系统的高催化作用。观察到,同时存在MoS 2 QD和GQD对罗丹明B(RB)-H 2 O 2反应的化学发光(CL)发射具有强大的增强作用。与每种组分相比,MoS 2 QD和GQDs混合物(以3:2的比例制备)显示出优异的催化活性。在CL发射强度和H之间获得线性关系2 O 2浓度在1.5–460 nmol L -1的范围内。另一方面,由于胆固醇的酶促氧化导致H 2 O 2的产生;检查提供的CL系统,以检测胆固醇被胆固醇氧化酶(ChOx)酶氧化后的胆固醇。在此,通过MoS 2纳米片实现了进一步的改进。MoS 2纳米片增强了ChOx在胆固醇氧化过程中的性能。获得的结果证实了在0.08–300 µmol L -1的线性动态范围内对胆固醇浓度的高度选择性和灵敏测定,检测限(3S)为35 nmol L -1。该方法成功用于人体血清中胆固醇水平的检测。

更新日期:2017-09-01
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