Developing a novel non-enzyme mimetic in biosensors is of great significance. Here, a synergetic peroxidase-like activity was disclosed for mixed MoS₂ quantum dots (MoS₂ QDs) and graphene quantum dots (GQDs). The high catalytic effect of this mixture was studied on the chemiluminescence system. It was observed that the simultaneous presence of MoS₂ QDs and GQDs had a powerful enhancing effect on the chemiluminescence (CL) emission of rhodamine B (RB)-H₂O₂ reaction. MoS₂ QDs and GQDs mixture (prepared with a ratio of 3:2) showed a superior catalytic activity when compared to each of the constituents. A linear relationship was acquired between the CL emission intensity and H₂O₂ concentration in the range of 1.5–460 nmol L⁻¹. On the other hand, since the enzymatic oxidation of cholesterol leads to the production of H₂O₂; the offered CL system was examined to detect cholesterol after its oxidation by cholesterol oxidase (ChOx) enzyme. Herein, a further improvement was achieved by MoS₂ nanosheets. The MoS₂ nanosheets increased the performance of ChOx in cholesterol oxidation process. The obtained results confirmed a highly selective and sensitive determination of cholesterol concentration in a linear dynamic range of 0.08–300 µmol L⁻¹, with a detection limit (3S) of 35 nmol L⁻¹. The developed method was successfully applied for the detection of cholesterol level in human serum samples.

在生物传感器中开发新型的非酶模拟物具有重要意义。在此，公开了对于混合的MoS ₂量子点（MoS ₂ QDs）和石墨烯量子点（GQDs）的协同过氧化物酶活性。研究了该混合物对化学发光系统的高催化作用。观察到，同时存在MoS ₂ QD和GQD对罗丹明B（RB）-H ₂ O ₂反应的化学发光（CL）发射具有强大的增强作用。与每种组分相比，MoS ₂ QD和GQDs混合物（以3：2的比例制备）显示出优异的催化活性。在CL发射强度和H之间获得线性关系₂ O ₂浓度在1.5–460 nmol L ^-1的范围内。另一方面，由于胆固醇的酶促氧化导致H ₂ O _2的产生；检查提供的CL系统，以检测胆固醇被胆固醇氧化酶（ChOx）酶氧化后的胆固醇。在此，通过MoS ₂纳米片实现了进一步的改进。MoS ₂纳米片增强了ChOx在胆固醇氧化过程中的性能。获得的结果证实了在0.08–300 µmol L ^-1的线性动态范围内对胆固醇浓度的高度选择性和灵敏测定，检测限（3S）为35 nmol L ^-1。该方法成功用于人体血清中胆固醇水平的检测。