In this tutorial we describe a fast, nondestructive, three-dimensional (3-D) view approach to be used in morphology characterization of capillary monoliths and columns by reconstruction from X-ray microtomography (XMT) obtained by acquiring projection images of the sample from a number of different directions. The method comprises imaging acquisition, imaging reconstruction using specific algorithms and imaging analysis by generation of a 3-D image of the sample from radiographic images. The 3-D images show the morphological data for bulk macropore space and skeleton connectivity of the monoliths and were compared with other images from imaging techniques such as scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and with chromatographic performance. The 3-D XMT methodology is applicable for organic and inorganic capillary chromatographic monolithic materials and it allows the acquisition of many hundreds (in our case 1001 projections) of longitudinal and cross-sectional images in a single session, resolving morphological details with a 3D-view of the monolithic structure, inclusive inside the column in a sectional structure with volume (three dimensions) when compared to the sectional structure area (with only two dimensions) when using SEM and FESEM techniques.

中文翻译：

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用于表征整体固定相和毛细管液相色谱柱的 3 维 X 射线显微断层扫描方法 - 教程

在本教程中，我们描述了一种快速、无损、三维 (3-D) 视图方法，该方法通过从 X 射线显微断层扫描 (XMT) 重建获得的样品投影图像，用于毛细管整体和柱的形态表征。许多不同的方向。该方法包括成像采集、使用特定算法的成像重建和通过从射线照相图像生成样本的 3-D 图像的成像分析。3-D 图像显示了整体大孔空间和骨架连通性的形态数据，并与来自扫描电子显微镜 (SEM) 和场发射扫描电子显微镜 (FESEM) 等成像技术的其他图像以及色谱性能进行了比较。