In effect-directed analysis (EDA) with high-performance thin-layer chromatography (HPTLC), the effect is often detected using images. Thus, an approach to create inhibition chromatograms from these images was developed using the example of the HPTLC- bioluminescence inhibition test. A comparison between the cuvette test and the HPTLC test shows that the test on the plate is significantly more sensitive. To describe the strength of the effect, the EC₅₀ value is determined from the dose-response relationship. However, the inhibiting compounds are generally unknown and thus their concentrations are also unknown. Therefore, instead of the concentration, the known application volumes are used. This enables the calculation of the application volume necessary to achieve 50% inhibition. Since the volume is inversely proportional to the concentration, the reciprocal value of the calculated volume is indicated and is referred to as the reciprocal iso-inhibition volume (RIV). Using this RIV-concept, it is now possible to compare inhibition bands within and between plates. The entire evaluation is described by the means of two samples from a contaminated site using the bioluminescence inhibition.

在使用高效薄层色谱（HPTLC）进行的效果导向分析（EDA）中，通常使用图像来检测效果。因此，使用HPTLC-生物发光抑制测试的实例开发了一种从这些图像创建抑制色谱图的方法。比色杯测试和HPTLC测试之间的比较表明，在板上进行的测试更加灵敏。为了描述效果的强度，EC ₅₀值由剂量-反应关系确定。然而，抑制性化合物通常是未知的，因此它们的浓度也是未知的。因此，代替浓度，使用已知的施用量。这样就可以计算出达到50％抑制作用所需的应用量。由于体积与浓度成反比，因此指示了计算体积的倒数，并称为倒数等抑制体积（RIV）。使用RIV概念，现在可以比较板内和板之间的抑制带。整个评估是通过使用生物发光抑制剂从两个受污染的地点采集的样品来描述的。