Profiling Protein S-Sulfination with Maleimide-Linked Probes,ChemBioChem

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Profiling Protein S-Sulfination with Maleimide-Linked Probes
ChemBioChem ( IF 2.6 ) Pub Date : 2017-09-01 01:20:52 , DOI: 10.1002/cbic.201700137
Yu-Hsuan Kuo ₁ , Aaron M. Konopko ₁ , Nicholas B. Borotto ₁ , Jaimeen D. Majmudar ₁ , Sarah E. Haynes ₁ , Brent R. Martin ₁

Affiliation

Maleimide, but not iodoacetamide, reacts with aryl and alkyl sulfinic acid standards and S-sulfinated proteins to give a sulfonyl-succinimide adduct that is stable under acidic conditions. This sequential alkylation strategy can be used for selective sulfinic acid labeling in biological samples. This study reveals a broadened profile of maleimide reactivity across cysteine modifications in proteins.

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