Current organ transplantation protocols require the rapid transport of freshly isolated donor tissue to the recipient patient at the site where the procedure is to be conducted. During transport, the tissue graft can quickly deteriorate as a result of oxygen starvation. In this study, we report the fabrication of oxygen-releasing coatings for improved tissue preservation. The coatings were prepared via the encapsulation of calcium peroxide or urea peroxide microparticles between layers of octadiene plasma polymer films. By varying the thickness of the plasma polymer coating and type of peroxide, formulations were obtained that generate oxygen upon contact with aqueous solutions, while at the same time limiting the amount of toxic reactive oxygen species produced. The optimized coatings were tested under hypoxic conditions using the MIN6 β-cell line, which resulted in a 3-fold increase in the viability of cultured cells. These thin oxygen-releasing coatings can be deposited on a wide range of surfaces, creating a platform for oxygen delivery with the potential to extend the viability of transported tissues and increase the time frame available for graft transport.

中文翻译：

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增氧涂层，改善组织保存

当前的器官移植方案要求将新鲜分离的供体组织在要进行该手术的部位快速运送到接受患者。在运输过程中，组织移植物会因缺氧而迅速退化。在这项研究中，我们报告了用于改善组织保存的氧气释放涂层的制造。通过将过氧化钙或过氧化脲微粒封装在辛二烯等离子体聚合物膜层之间来制备涂层。通过改变等离子体聚合物涂层的厚度和过氧化物的类型，获得了在与水溶液接触时产生氧气的配方，同时限制了所产生的有毒活性氧的数量。在缺氧条件下，使用MIN6β细胞系对优化的涂层进行了测试，这导致培养细胞的活力提高了3倍。这些薄薄的氧气释放涂层可以沉积在广泛的表面上，从而为氧气输送创造了平台，从而有可能延长被输送组织的生存能力并增加可用于移植物运输的时间框架。