Achievement of an endothelial cell (EC) monolayer (re-endothelialization) on the vascular implant surface with competent and functioning features is critical for long-term safety after implantation. Oriented EC monolayer is beneficial to improve endothelial function such as enhanced athero-resistant property. However, the information about antithrombotic property of oriented EC monolayer is limited. Here, we used the microgrooved polydimethylsiloxane substrates to guide EC orientation and obtain oriented EC monolayer. The effects of anisotropic topography on EC behaviors and antithrombotic function of the EC monolayer were then evaluated. Our data demonstrated that ECs responded to grooves in a size-dependent way as shown in oriented cell cytoskeleton and nuclei, enhanced directed migration, and overall velocity. Furthermore, compared to the EC monolayer on the flat surface, the oriented EC monolayer formed on the grooved substrates exhibited improved antithrombotic capability as indicated by higher expression of functional related genes, production of prostacyclin and tissue plasminogen activator, and prolonged activated coagulation time. The improvement of antithrombotic function was especially notable on the smaller-size groove. These findings reveal the responses of ECs to varisized topography and antithrombotic function of the oriented EC monolayer, providing insights into optimal design of vascular implants.

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定向内皮细胞单层对微槽的抗血栓功能的改善

在血管植入物表面上具有功能和功能特征的内皮细胞（EC）单层（再内皮化）的实现对于植入后的长期安全性至关重要。定向的EC单层有利于改善内皮功能，例如增强的抗动脉粥样硬化性能。但是，有关定向EC单层的抗血栓形成特性的信息是有限的。在这里，我们使用了微刻槽的聚二甲基硅氧烷基材来引导EC取向并获得取向的EC单层。然后评估各向异性地形对EC行为和EC单层抗血栓形成功能的影响。我们的数据表明，ECs以大小依赖性方式响应沟，如定向细胞的细胞骨架和细胞核，增强的定向迁移和整体速度所示。此外，与平坦表面上的EC单分子层相比，形成在带凹槽的基材上的定向EC单分子层具有更高的抗血栓形成能力，这可以通过功能相关基因的更高表达，前列环素和组织纤溶酶原激活剂的产生以及延长的活化凝血时间来表明。在较小尺寸的凹槽上，抗血栓功能的改善尤为明显。这些发现揭示了ECs对定向EC单层的各种形貌和抗血栓形成功能的反应，从而为血管植入物的最佳设计提供了见识。并延长活化凝血时间。在较小尺寸的凹槽上，抗血栓功能的改善尤为明显。这些发现揭示了ECs对定向EC单层的各种形貌和抗血栓形成功能的反应，从而为血管植入物的最佳设计提供了见识。并延长活化凝血时间。在较小尺寸的凹槽上，抗血栓功能的改善尤为明显。这些发现揭示了ECs对定向EC单层的各种形貌和抗血栓形成功能的反应，从而为血管植入物的最佳设计提供了见识。