Poly(propylene fumarate) (PPF) has been highlighted as one of the most promising materials for bone regeneration. Despite the promising advantages of using polymer scaffolds for biomedical applications, their inherent lack of bioactivity has limited their clinical application. In this study, PPF was successfully functionalized with Bioglass and a novel catechol-bearing peptide bioconjugate containing bioactive short peptide sequences of basic fibroblast growth factor, bone morphogenetic protein 2, and osteogenic growth peptide. The binding affinity was assessed to be around 110 nmol/cm² with the Bioglass content at 10 wt %. Fluorescence imaging studies show that the catechol-bearing modular peptide binds preferentially to the Bioglass. A 4 week in vitro cell study using human mesenchymal stem cells showed that cell adhesion, spreading, proliferation, and osteogenic differentiation at both gene and protein levels were all improved by the introduction of peptides, demonstrating the potential approach of dually functionalized polymers for bone regeneration.

中文翻译：

---

用肽修饰聚富马酸丙二醇酯-生物玻璃复合材料以增强生物活性

聚富马酸丙二酯（PPF）已被强调为最有前途的骨再生材料之一。尽管将聚合物支架用于生物医学应用具有令人鼓舞的优势，但它们固有的缺乏生物活性限制了其临床应用。在这项研究中，PPF已成功地用Bioglass和一种新型的含有邻苯二酚的肽生物缀合物进行了功能化，该生物缀合物包含碱性成纤维细胞生长因子，骨形态发生蛋白2和成骨生长肽的生物活性短肽序列。结合亲和力被评估为约110nmol / cm ^2。生物玻璃含量为10 wt％。荧光成像研究表明，带有邻苯二酚的模块化肽优先与生物玻璃结合。使用人间充质干细胞进行的为期4周的体外细胞研究表明，通过引入肽可以改善基因和蛋白质水平上的细胞粘附，扩散，增殖和成骨分化，这证明了双重功能化聚合物用于骨再生的潜在方法。