Amyloid fibrils prepared from β-lactoglobulin were used to form freeze-dried and cross-linked aerogels. By varying the fibril concentration and freezing gradient, it was possible to control the pore structure and elastic modulus of the aerogels within one order of magnitude from ∼20 to ∼200 kPa. Using butane tetracarboxylic acid as cross-linker, these aerogels maintained their monolithic shape under aqueous conditions, displaying elastic behavior and a modulus in the range of ∼4–40 kPa. When explored as scaffolds for cell growth, the amyloid fibril aerogels demonstrated biocompatibility and led to the successful penetration and permeation of two epithelial cell lines (Caco-2 and HT29) throughout the scaffold. These soft, elastic, and water-stable biomaterials expand the scope of amyloid fibril aerogels, making them suitable for wet-state applications such as heterogeneous catalysis, purification membranes, and 3D matrices for cell growth.

中文翻译：

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冰模板和交联的淀粉样原纤维气凝胶支架，用于细胞生长。

由β-乳球蛋白制备的淀粉样原纤维用于形成冻干和交联的气凝胶。通过改变原纤维浓度和冻结梯度，可以将气凝胶的孔结构和弹性模量控制在20〜200 kPa的一个数量级内。使用丁烷四羧酸作为交联剂，这些气凝胶在水性条件下保持其整体形状，显示出弹性行为，模量在约4–40 kPa范围内。当探索为细胞生长的支架时，淀粉样蛋白原纤维气凝胶表现出生物相容性，并导致两种上皮细胞系（Caco-2和HT29）成功渗透并渗透整个支架。这些柔软，弹性和耐水的生物材料扩大了淀粉样蛋白原纤维气凝胶的范围，