Mating in the Closest Living Relatives of Animals Is Induced by a Bacterial Chondroitinase.,Cell

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Mating in the Closest Living Relatives of Animals Is Induced by a Bacterial Chondroitinase.
Cell ( IF 45.5 ) Pub Date : 2017-Sep-07 , DOI: 10.1016/j.cell.2017.08.005
Arielle Woznica ₁ , Joseph P Gerdt ₂ , Ryan E Hulett ₁ , Jon Clardy ₂ , Nicole King ₁

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We serendipitously discovered that the marine bacterium Vibrio fischeri induces sexual reproduction in one of the closest living relatives of animals, the choanoflagellate Salpingoeca rosetta. Although bacteria influence everything from nutrition and metabolism to cell biology and development in eukaryotes, bacterial regulation of eukaryotic mating was unexpected. Here, we show that a single V. fischeri protein, the previously uncharacterized EroS, fully recapitulates the aphrodisiac-like activity of live V. fischeri. EroS is a chondroitin lyase; although its substrate, chondroitin sulfate, was previously thought to be an animal synapomorphy, we demonstrate that S. rosetta produces chondroitin sulfate and thus extend the ancestry of this important glycosaminoglycan to the premetazoan era. Finally, we show that V. fischeri, purified EroS, and other bacterial chondroitin lyases induce S. rosetta mating at environmentally relevant concentrations, suggesting that bacteria likely regulate choanoflagellate mating in nature.

中文翻译：

细菌软骨素酶在动物的近亲中交配。

我们偶然发现，海洋细菌费氏弧菌会在动物的最亲近的亲戚之一-鞭毛鞭毛藻（Salpingoeca rosetta）中诱导有性繁殖。尽管细菌影响着从营养，代谢到细胞生物学以及真核生物发育的各个方面，但对真核生物交配的细菌调控却是出乎意料的。在这里，我们显示了一个单一的费氏弧菌蛋白，即以前未表征的EroS，完全概括了活费氏弧菌的类似壮阳药的活性。EroS是一种软骨素裂解酶；尽管其底物硫酸软骨素以前被认为是动物的突触，但我们证明了罗塞塔氏菌产生硫酸软骨素，因此将这种重要的糖胺聚糖的祖先扩展到了前甲唑烷时代。最后，我们证明了费氏弧菌，纯化的EroS，

更新日期：2017-08-31

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