An efficient high-performance countercurrent chromatography (HPCCC) based method has been developed for the purification of chemically synthesized 1-adenosin-5′-yl 3-(3-methylbut-3-enyl)triphosphoric acid diester (ApppI). ApppI is an adenosine triphosphate (ATP) analogue with biological significance due to its varied actions in the body. ApppI was synthesized and purified as its tetrabutylammonium (TBA) salt and converted successfully into its more practical sodium salt form after purification. The amount of TBA hydroxide (2.0, 2.5 and 3.0 eq) used in the synthesis of ApppI was shown to exert an effect on the purification process with HPCCC and on the overall yield (8%, 16% and 22%, respectively). 1-Adenosin-5′-yl 3-(3-methylbut-3-enyl)diphosphoric acid diester (AppI) was also isolated as a side product.

已经开发了一种基于高效高性能逆流色谱（HPCCC）的方法，用于纯化化学合成的1-腺苷-5'-基3-（3-甲基丁-3-烯基）三磷酸二酯（ApppI）。ApppI是一种三磷酸腺苷（ATP）类似物，由于其在体内的多种作用而具有生物学意义。合成并纯化的ApppI为四丁基铵（TBA）盐，纯化后成功转化为更实用的钠盐形式。已显示，在ApppI的合成中使用的TBA氢氧化物的量（2.0、2.5和3.0 eq）对使用HPCCC的纯化过程和总收率（分别为8％，16％和22％）产生影响。还分离了作为副产物的1-腺苷-5'-基3-（3-甲基丁-3-烯基）二磷酸二酯（AppI）。