The substitution of ewe milk with more economic cow milk is a common fraud. Here we present a capillary electrophoresis method for the quantification of ewe milk in ovine/bovine milk mixtures, which allows for the rapid and inexpensive recognition of ewe milk adulteration with cow milk. We utilized a routine CE method for human blood and urine proteins analysis, which fulfilled the separation of skimmed milk proteins in alkaline buffer. Under this condition, ovine and bovine milk exhibited a recognizable and distinct CE protein profiles, with a specific ewe peak showing a reproducible migration zone in ovine/bovine mixtures. Based on ewe specific CE peak, we developed a method for ewe milk quantification in ovine/bovine skimmed milk mixtures, which showed good linearity, precision and accuracy, and a minimum amount of detectable fraudulent cow milk equal to 5%.

用更经济的牛奶代替母乳是普遍的欺诈行为。在这里，我们介绍了一种用于定量羊/牛乳混合物中母乳的毛细管电泳方法，该方法可快速，廉价地识别母乳与牛奶的掺假。我们利用常规的CE方法对人体血液和尿液中的蛋白质进行分析，从而实现了碱性缓冲液中脱脂牛奶蛋白质的分离。在这种条件下，羊奶和牛乳表现出可识别的独特的CE蛋白谱，特定的母羊峰显示出在羊/牛混合物中可再现的迁移区。根据母羊特定的CE峰，我们开发了一种定量羊/牛脱脂奶混合物中母乳的方法，该方法显示出良好的线性，精密度和准确性，