A pyrene-degrading microbial consortium was obtained after enrichment with mangrove sediment collected from Thailand. Five cultivable bacteria (Mycobacterium spp. PO1 and PO2, Novosphingobium pentaromativorans PY1, Ochrobactrum sp. PW1, and Bacillus sp. FW1) were successfully isolated from the consortium. Draft genomes of them showed that two different morphotypes of Mycobacterium (PO1 and PO2), possessed a complete gene set for pyrene degradation. PY1 contained genes for phthalate assimilation via protocatechuate, a central intermediate, by meta-cleavage pathway, and PW1 possessed genes for protocatechuate degradation via ortho-cleavage pathway. The occurrence of biosurfactant-producing genes in FW1 suggests the involvement in enhancing the pyrene bioavailability. Biotransformation experiments revealed that Mycobacterium completely degraded 100 mg L⁻¹ pyrene within six days, whereas no significant degradation was observed with the others. Notably, PY1 and PW1 exhibited higher activity for protocatechuate degradation than the others. The artificially reconstructed consortia containing Mycobacterium with the other three strains (PY1, PW1 and FW1) showed three-fold higher degradation rate for pyrene than the individual Mycobacterium. The enhanced pyrene biodegradation achieved in the consortium was due to the cooperative interaction of bacterial mixture. Our findings showing that synergistic degradation of pyrene in the consortium will facilitate the application of the defined bacterial consortium in bioremediation.

中文翻译：

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红树林沉积物来源的细菌群落中五种可培养细菌协同降解of

富含从泰国收集的红树林沉积物后，获得了可降解pyr的微生物财团。从该财团中成功分离出五种可培养细菌（分枝杆菌属PO1和PO2，新孢子虫PY1，Ochrobactrum sp。PW1和Bacillus sp。FW1）。他们的基因组草图显示，分枝杆菌的两种不同形态类型（PO1和PO2）具有完整的for降解基因组。PY1经由儿茶酸包含基因邻苯二甲酸酯同化，中央中间，由元-cleavage途径和PW1具有基因用于经由儿茶酸降解邻裂解途径。FW1中生物表面活性剂生产基因的出现表明参与the生物利用度的提高。生物转化实验表明，分枝杆菌在六天内完全降解了100 mg L ^-1 pyr，而其他分枝杆菌未见明显降解。值得注意的是，PY1和PW1对原儿茶酸的降解表现出比其他更高的活性。包含分枝杆菌与其他三种菌株（PY1，PW1和FW1）的人工重建的联合体显示，pyr的降解速率比单个分枝杆菌高三倍。财团中增强的pyr生物降解作用归因于细菌混合物的协同相互作用。我们的发现表明，degradation在联合体中的协同降解将促进确定的细菌联合体在生物修复中的应用。