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A miniature liquid electrode discharge-optical emission spectrometric system integrating microelectrodialysis for potassium screening in serum†
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2017-05-05 00:00:00 , DOI: 10.1039/c7ja00111h Shuang Liu 1, 2, 3, 4, 5 , Yi Cai 1, 2, 3, 4, 5 , Yong-Liang Yu 1, 2, 3, 4, 5 , Jian-Hua Wang 1, 2, 3, 4, 5
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2017-05-05 00:00:00 , DOI: 10.1039/c7ja00111h Shuang Liu 1, 2, 3, 4, 5 , Yi Cai 1, 2, 3, 4, 5 , Yong-Liang Yu 1, 2, 3, 4, 5 , Jian-Hua Wang 1, 2, 3, 4, 5
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An automated microelectrodialysis (μED)-liquid electrode discharge (LED)-optical emission spectrometric (OES) system was developed with the aim of potassium screening in serum, especially for a micro-sample volume. Potassium in serum was on-line extracted via a μED process and subsequently introduced into the LED microplasma to perform the optical emission detection at λem 766.5 nm. The optical emission intensity of potassium was measured using a charge-coupled device (CCD) spectrometer for quantitative analysis. A few important parameters governing the extraction of potassium by μED and its detection by LED-OES were investigated. The present system provides an excellent capability for eliminating matrix interference with a protein removal efficiency of >99%, by consuming a serum sample volume of 20 μL for each analysis. The entire analytical process, including sample introduction, pretreatment and detection, takes no more than 90 s. A detection limit of 0.6 mg L−1 was obtained along with a linear range of 2–70 mg L−1 and a RSD value of 4.8% at 5 mg L−1 potassium. The practical applicability of the μED-LED-OES system was demonstrated by the determination of potassium in serum certified reference materials and real human serum samples. The present system offers a potential strategy for screening the variation of the concentration of potassium in patients.
中文翻译：
集成微电渗析的微型液体电极放电-光发射光谱系统，用于血清钾筛选†
自动化微电渗析（μED）-液体电极放电（LED）-光发射光谱（OES）系统的开发旨在对血清中的钾进行筛查，特别是对于微量样品。钾在血清是上线提取经由一个μED过程，并随后引入到LED微等离子体在执行光发射检测λ EM766.5 nm。使用电荷耦合器件（CCD）光谱仪测量钾的光发射强度，以进行定量分析。研究了一些重要的参数，这些参数决定了用μED萃取钾和用LED-OES检测钾的能力。本系统通过每次分析消耗20μL的血清样品量，具有消除基质干扰的出色能力，蛋白质去除效率> 99％。整个分析过程，包括样品引入，预处理和检测，不超过90 s。检出限为0.6 mg L -1，线性范围为2–70 mg L -1，在5 mg L -1时的RSD值为4.8％钾。μED-LED-OES系统的实际适用性通过测定血清认证参考物质和真实人类血清样品中的钾来证明。本系统提供了用于筛选患者中钾浓度变化的潜在策略。
更新日期：2017-05-05
中文翻译：
集成微电渗析的微型液体电极放电-光发射光谱系统，用于血清钾筛选†
自动化微电渗析（μED）-液体电极放电（LED）-光发射光谱（OES）系统的开发旨在对血清中的钾进行筛查，特别是对于微量样品。钾在血清是上线提取经由一个μED过程，并随后引入到LED微等离子体在执行光发射检测λ EM766.5 nm。使用电荷耦合器件（CCD）光谱仪测量钾的光发射强度，以进行定量分析。研究了一些重要的参数，这些参数决定了用μED萃取钾和用LED-OES检测钾的能力。本系统通过每次分析消耗20μL的血清样品量，具有消除基质干扰的出色能力，蛋白质去除效率> 99％。整个分析过程，包括样品引入，预处理和检测，不超过90 s。检出限为0.6 mg L -1，线性范围为2–70 mg L -1，在5 mg L -1时的RSD值为4.8％钾。μED-LED-OES系统的实际适用性通过测定血清认证参考物质和真实人类血清样品中的钾来证明。本系统提供了用于筛选患者中钾浓度变化的潜在策略。
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