Dual, Site-Specific Modification of Antibodies by Using Solid-Phase Immobilized Microbial Transglutaminase,ChemBioChem

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Dual, Site-Specific Modification of Antibodies by Using Solid-Phase Immobilized Microbial Transglutaminase
ChemBioChem ( IF 2.6 ) Pub Date : 2017-08-29 11:15:42 , DOI: 10.1002/cbic.201700188
Philipp R. Spycher ₁ , Christian A. Amann ₁ , Jöri E. Wehrmüller ₁ , David R. Hurwitz ₁ , Olivier Kreis ₁ , Daniel Messmer ₂ , Andreas Ritler _{1,

3} , Andreas Küchler ₂ , Alain Blanc ₁ , Martin Béhé ₁ , Peter Walde ₂ , Roger Schibli _{1,

4}

Affiliation

Double take: Solid-phase immobilized microbial transglutaminase (MTG) enables the site-specific modification of glutamine and/or lysine residues of various proteins with probes suitable for bioorthogonal click reactions. Site-specific, dual modification of antibodies is demonstrated with chemical entities suitable for dual-imaging applications without affecting antibody–antigen binding.

中文翻译：

通过使用固相固定微生物转谷氨酰胺酶对抗体进行双重，特定于位点的修饰

双重建议：固相固定的微生物转谷氨酰胺酶（MTG）可使用适合于生物正交点击反应的探针对各种蛋白质的谷氨酰胺和/或赖氨酸残基进行位点特异性修饰。通过适用于双重成像应用的化学实体证明了抗体的位点特异性，双重修饰，而不会影响抗体与抗原的结合。

更新日期：2017-08-29

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