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Assessment on cadmium and lead in soil based on a rhizosphere microbial community
Toxicology Research ( IF 2.2 ) Pub Date : 2017-06-16 00:00:00 , DOI: 10.1039/c7tx00048k
Xu Zhang 1, 2, 3, 4 , Huanhuan Yang 2, 3, 4, 5 , Zhaojie Cui 1, 2, 3, 4
Affiliation  

The soil ecosystem is easily polluted by heavy metals. Cadmium (Cd) and lead (Pb), as the main pollutants of heavy metals, cause much harm to the soil ecosystem. However, the impact of the two chemicals on rhizosphere microorganisms remains almost unknown. The change of catalase (CAT) activity was consistent with the microbial biomass. 16S rRNA gene sequencing was performed on soil samples to study the toxic effect of heavy metals. On performing sequence analysis at the phylum and family taxonomic levels, 32 identified phyla and 303 families were observed. The dominant phylum was Proteobacteria followed by Bacteroidetes, Acidobacteria, and Actinobacteria. The relative abundance of the dominant phyla was obviously changed under the stress of Cd and Pb, suggesting that the heavy metal input had affected the microbial community structure. At the Order and Family levels, there was different variation of richness and diversity in Cd and Pb group as compared to those in the control group. Furthermore, abundance and similarity analysis showed the differences between Cd and Pb, indicating different toxicology effect on rhizosphere microbial communities because of the unique properties. This study provided a novel insight into the composition of microbial communities of rhizosphere, which could be used to evaluate the soil environment.

中文翻译:

基于根际微生物群落的土壤中镉和铅评估

土壤生态系统很容易被重金属污染。镉(Cd)和铅(Pb)作为重金属的主要污染物,对土壤生态系统造成了很大的危害。但是,这两种化学物质对根际微生物的影响仍然未知。过氧化氢酶(CAT)活性的变化与微生物生物量一致。对土壤样品进行了16S rRNA基因测序,以研究重金属的毒性作用。在门和家庭分类水平上进行序列分析时,观察到32个已鉴定门和303个家庭。优势门是Proteobacteria,其次是拟杆菌,Acidobacteria和Actinobacteria。在Cd和Pb胁迫下,优势种的相对丰度发生了明显变化,表明重金属的输入已经影响了微生物群落结构。在Corder和Family水平上,Cd和Pb组的丰富度和多样性与对照组相比有所不同。此外,丰度和相似性分析显示了镉和铅之间的差异,这表明由于其独特的性质,其对根际微生物群落的毒理作用不同。这项研究提供了对根际微生物群落组成的新见解,可用于评估土壤环境。
更新日期:2017-06-16
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