Engineering scaffolds with excellent electro-activity are increasingly important to tissue engineering and regenerative medicine. Herein, conductive poly(3,4-ethylenedioxythiophene) doped with hyaluronic acid (PEDOT-HA) nanoparticles were firstly synthesized via chemical oxidant polymerization. Three-dimension (3D) PEDOT-HA/Cs/Gel scaffold was then developed by introducing PEDOT-HA nanoparticles into the chitosan/gelatin (Cs/Gel) matrix. HA, as a bridge, not only was used as a dopant, but also combined PEDOT into the Cs/Gel via chemical crosslinking. The PEDOT-HA/Cs/Gel scaffold was used as a conductive substrate for neural stem cells (NSCs) culture in vitro. The results demonstrated PEDOT-HA/Cs/Gel scaffold had excellent biocompatibility for NSCs proliferation and differentiation. 3D confocal fluorescence images showed cells attached on the channel surface of Cs/Gel and PEDOT-HA/Cs/Gel scaffold with normal neuronal morphology. Compared to Cs/Gel scaffold, PEDOT-HA/Cs/Gel scaffold not only promoted NSCs proliferation with up-regulated expression of Ki67, but also enhanced NSC differentiation into neurons and astrocytes with up-regulated expression of β tubulin-III and GFAP, respectively. It is expected that this electro-active and bio-active PEDOT-HA/Cs/Gel scaffold will be used as a conductive platform to regulate NSCs behaviors for neural tissue engineering.

中文翻译：

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导电性PEDOT-HA / Cs / Gel支架中神经干细胞在神经组织工程中的增殖和分化

具有出色电活性的工程支架对组织工程和再生医学越来越重要。在此，首先通过化学氧化剂聚合合成了透明质酸（PEDOT-HA）纳米粒子掺杂的导电聚（3,4-乙撑二氧噻吩）。然后通过将PEDOT-HA纳米粒子引入壳聚糖/明胶（Cs / Gel）基质中来开发三维（3D）PEDOT-HA / Cs /凝胶支架。HA，作为桥，不仅用作掺杂剂，而且还通过化学交联将PEDOT结合到Cs / Gel中。PEDOT-HA / Cs / Gel支架用作神经干细胞（NSCs）体外培养的导电底物。结果表明，PEDOT-HA / Cs / Gel支架对于NSC的增殖和分化具有极好的生物相容性。3D共聚焦荧光图像显示细胞附着在具有正常神经元形态的Cs / Gel和PEDOT-HA / Cs / Gel支架的通道表面上。与Cs / Gel支架相比，PEDOT-HA / Cs / Gel支架不仅可以通过上调Ki67的表达来促进NSC的增殖，还可以通过上调β微管蛋白III和GFAP的表达来增强NSC向神经元和星形胶质细胞的分化，分别。可以预期，这种电活性和生物活性的PEDOT-HA / Cs / Gel支架将用作调节神经组织工程中NSCs行为的导电平台。而且分别通过上调β微管蛋白III和GFAP的表达来增强NSC向神经元和星形胶质细胞的分化。预期这种电活性和生物活性的PEDOT-HA / Cs / Gel支架将用作调节神经组织工程中NSCs行为的传导平台。而且分别通过上调β微管蛋白III和GFAP的表达来增强NSC向神经元和星形胶质细胞的分化。可以预期，这种电活性和生物活性的PEDOT-HA / Cs / Gel支架将用作调节神经组织工程中NSCs行为的导电平台。