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A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities.
Nature Microbiology ( IF 20.5 ) Pub Date : 2017-Oct-01 , DOI: 10.1038/s41564-017-0010-9
Bennett S. Lambert , Jean-Baptiste Raina , Vicente I. Fernandez , Christian Rinke , Nachshon Siboni , Francesco Rubino , Philip Hugenholtz , Gene W. Tyson , Justin R. Seymour , Roman Stocker

Microbial interactions influence the productivity and biogeochemistry of the ocean, yet they occur in miniscule volumes that cannot be sampled by traditional oceanographic techniques. To investigate the behaviours of marine microorganisms at spatially relevant scales, we engineered an in situ chemotaxis assay (ISCA) based on microfluidic technology. Here, we describe the fabrication, testing and first field results of the ISCA, demonstrating its value in accessing the microbial behaviours that shape marine ecosystems.

中文翻译:

基于微流控技术的原位趋化分析,以研究水生微生物群落的行为。

微生物相互作用影响海洋的生产力和生物地球化学,但它们的体积很小,而传统海洋学技术无法对其进行采样。为了研究空间相关尺度上海洋微生物的行为,我们设计了一种基于微流体技术的原位趋化性测定法(ISCA)。在这里,我们描述了ISCA的制造,测试和第一现场结果,展示了ISCA在获取影响海洋生态系统的微生物行为方面的价值。
更新日期:2017-08-28
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